Skip to main content
SpringerLink
Log in
Book cover

Clinical and Preclinical Models for Maximizing Healthspan

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Paul C. Guest

    1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2138)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (32 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Reviews

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Impact of New Biomarkers and Drug Targets on Age-Related Disorders

      • Paul C. Guest
      Pages 3-28

    3. Vitamin D and Muscle Sarcopenia in Aging

      • Behnaz Abiri, Mohammadreza Vafa
      Pages 29-47

    4. Quantitative Assays of Plasma Apolipoproteins

      • Anne Poljak, Mark W. Duncan, Tharusha Jayasena, Perminder S. Sachdev
      Pages 49-81

    5. Protocol for the Use of the Ketogenic Diet in Preclinical and Clinical Practice

      • Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai
      Pages 83-98

  3. Protocols

    1. Front Matter

      Pages 99-99
      Download chapter PDF

    2. An In Vivo/Ex Vivo Study Design to Investigate Effects of Chronic Conditions and Therapeutic Compounds on Adipose Stem Cells in Animal Models

      • Hanél Sadie-Van Gijsen, Liske Kotzé-Hörstmann, Barbara Huisamen
      Pages 101-118

    3. Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations

      • Yashar Mehrbani Azar, Maria Jacoba Kruger, Dalene de Swardt, Michelle Maartens, Ascentia Mathapelo Seboko, William Frank Ferris et al.
      Pages 119-134

    4. Investigating Alcohol Behavior and Physiology Using Drosophila melanogaster

      • Aliza K. De Nobrega, Kristine V. Luz, Katherine N. Lyons, Lisa C. Lyons
      Pages 135-158

    5. Using Genome-Editing Tools to Develop a Novel In Situ Coincidence Reporter Assay for Screening ATAD3A Transcriptional Inhibitors

      • Liwei Lang, Yong Teng
      Pages 159-166

    6. Visualizing and Evaluating Cancer Cell Growth and Invasion by a Novel 3D Culture System

      • Chloe Shay, Liwei Lang, Yong Teng
      Pages 167-173

    7. Exploiting Plug-and-Play Electrochemical Biosensors to Determine the Role of FGF19 in Sorafenib-Mediated Superoxide and Nitric Oxide Production in Hepatocellular Carcinoma Cells

      • Lixia Gao, Yong Teng
      Pages 175-183

    8. Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines

      • Chareeporn Akekawatchai, Sittiruk Roytrakul, Narumon Phaonakrop, Janthima Jaresitthikunchai, Sarawut Jitrapakdee
      Pages 185-193

    9. Real-Time PCR Analysis of Metabolism-Related Genes in a Long-Lived Model of C. elegans

      • Sumino Yanase
      Pages 195-205

    10. Clinical Assessment of the NADome as Biomarkers for Healthy Aging

      • Tharusha Jayasena, Sonia Bustamante, James Clement, Robert Welschinger, Gideon A. Caplan, Perminder S. Sachdev et al.
      Pages 207-216

    11. Two-Dimensional Gel Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Eye Lens to Identify Biomarkers of Age-Related Cataract

      • Paul C. Guest
      Pages 217-231

    12. Testing the Effects of Dietary Seafood Consumption on Depressive Symptoms

      • Maximus Berger, G. Paul Amminger, Robyn McDermott, Paul C. Guest, Zoltán Sarnyai
      Pages 233-242

    13. Assays for Monitoring the Effects of Nicotinamide Supplementation on Mitochondrial Activity in Saccharomyces cerevisiae

      • Ivan Orlandi, Marina Vai
      Pages 243-250

    14. Measurement of a Surrogate Biomarker for Arginine Vasopressin Secretion in Association with Physiometric and Molecular Biomarkers of Aging

      • Paul C. Guest, Hans-Gert Bernstein, Henrik Dobrowolny, Katrin Borucki, Sabine Westphal, Johann Steiner
      Pages 251-262
Back to top

Keywords

About this book

This book presents a series of protocols in multiple disease areas affected by the aging process along with several methods which have shown progress in nutrient- or intervention-based approaches to maximize the healthspan. Of interest to researchers in the areas of chronic disease, gerontology, physical exercise, and nutrition as well as to clinical scientists, physicians, and pharmacologists, this volume also provides important information on disease mechanisms and novel drug targets as each protocol will be presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Clinical and Preclinical Models forMaximizing Healthspan: Methods and Protocols is an ideal guide for preclinical and clinical researchers working toward the discoveries needed to lead to a world society in which individuals are not only living longer lives but more productive and healthier ones.

Editors and Affiliations

  • Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation