Coronavirus Replication and Reverse Genetics

  • Luis Enjuanes

Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 287)

Table of contents

  1. Front Matter
    Pages I-IX
  2. D. A. Brian, R. S. Baric
    Pages 1-30
  3. S. G. Sawicki, D. L. Sawicki
    Pages 31-55
  4. J. Ziebuhr
    Pages 57-94
  5. P. S. Masters, P. J. M. Rottier
    Pages 133-159
  6. L. Enjuanes, I. Sola, S. Alonso, D. Escors, S. Zúñiga
    Pages 161-197
  7. Back Matter
    Pages 253-257

About this book


Coronaviruses are the RNA viruses with the largest genome known to date (27 to 32 kb). Members of this virus family affect most domestic animal species, causing important socio-economical losses, and also infect humans. Human coronaviruses were known to cause the winter common cold, a mild infection without important pathological consequences except in immuno-compromised patients. Recently, two new human coronaviruses have emerged, one causing the Severe and Acute Respiratory Syndrome (SARS) that infected more than 8000 individuals, leading to more than 800 deaths in 32 countries. This epidemic mobilized the World Health Organization, which launched travel restrictions to certain parts of the world for the first time in the last 50 years. The fact that coronaviruses, as many other viruses, crossed the species barrier to infect humans has posed a serious challenge to scientists involved in animal and human health. Control of coronavirus-induced diseases can only be the consequence of research on virus molecular biology and pathogenesis. This book contains information on virus genome structure, mechanism of replication and transcription, and the development of tools that make possible reverse genetic studies to understand virus-host interactions and the molecular basis of virus pathogenesis. The book also provides essential information for the development of classical and recombinant vaccines to control coronavirus infections.


RNA Transcription-Regulating Sequences Transmissible Gastroenteritis Coronavirus diseases gene gene expression genetics genome structure hepatitis infection infections protein proteins vaccine virus

Editors and affiliations

  • Luis Enjuanes
    • 1
  1. 1.Department of Molecular and Cell BiologyCentro Nacional de BiotecnologíaMadridSpain

Bibliographic information

Industry Sectors
Health & Hospitals