Topics in Fluorescence Spectroscopy

Volume 5: Nonlinear and Two-Photon-Induced Fluorescence

  • Joseph R. Lakowicz

Part of the Topics in Fluorescence Spectroscopy book series (TIFS, volume 5)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Carey K. Johnson, Chaozhi Wan
    Pages 43-85
  3. Joseph R. Lakowicz, Ignacy Gryczynski
    Pages 87-144
  4. B. Van der Wieb Meer, S.-Y. Simon Chen
    Pages 145-185
  5. Borys Kierdaszuk, Ignacy Gryczynski, Joseph R. Lakowicz
    Pages 187-209
  6. G. J. Blanchard
    Pages 253-303
  7. Joseph R. Lakowicz, Ignacy Gryczynski
    Pages 305-360
  8. P. T. C. So, C. Y. Dong, K. M. Berland, T. French, E. Gratton
    Pages 427-469
  9. Back Matter
    Pages 541-544

About this book


Fluorescence spectroscopy continues its advance to more sophisticated methods and applications. As one looks over the previous decades, its appears that the first practical instruments for time-resolved measurements appeared in the 1970’s. The instrumentation and analysis methods for time-resolved fluorescence advanced rapidly throughout the 1980’s. Since 1990 we have witnessed a rapid migration of the principles of time-resolved fluorescence to cell biology and clinical appli- tions. Most recently, we have seen the introduction of multi-photon excitation, pump-probe and stimulated emission methods for studies of biological mac- molecules and for cellular imaging. These advanced topics are the subject of the present volume. Two-photon excitation was first predicted by Maria Goppert-Mayer in 1931, but was not experimentally observed until 1961. Observation of two-photon excitation required the introduction of lasers which provided adequate photon density for multi-photon absorption. Since the early observations of two-photon excitation in the 1960s, multi-photon spectroscopy has been limited to somewhat exotic applications of chemical physics, where it is used to study the electronic symmetry of small molecules. Placing one’s self back in 1980, it would be hard to imagine the use of multi-photon excitation in biophysics or cellular imaging.


Absorption Sorption fluorescence fluorescence spectroscopy membrane microscopy molecule photon protein quality quenching spectroscopy

Editors and affiliations

  • Joseph R. Lakowicz
    • 1
  1. 1.Center for Fluorescence Spectroscopy and Department of Biochemistry and Molecular BiologyUniversity of Maryland School of MedicineBaltimoreUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2002
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-306-45553-7
  • Online ISBN 978-0-306-47070-7
  • Buy this book on publisher's site
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