Genetic Engineering: Principles and Methods

Principles and Methods

  • Jane K. Setlow

Part of the Genetic Engineering book series (GEPM, volume 23)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Milton H. Saier Jr.
    Pages 1-10
  3. Collin C. Q. Vu, John A. Cidlowski
    Pages 11-33
  4. Kerri A. Mowen, Michael David
    Pages 35-44
  5. Pamela Sears, Thomas Tolbert, Chi-Huey Wong
    Pages 45-68
  6. Herbert P. Schweizer, Tung T. Hoang, Katie L. Propst, Henry R. Ornelas, RoxAnn R. Karkhoff-Schweizer
    Pages 69-81
  7. Timothy L. Tellinghuisen, Rushika Perera, Richard J. Kuhn
    Pages 83-112
  8. Geoffrey P. Lin Cereghino, Anthony J. Sunga, Joan Lin Cereghino, James M. Cregg
    Pages 157-169
  9. Izabela Giriat, Thomas W. Muir, Francine B. Perler
    Pages 171-199
  10. Back Matter
    Pages 221-231

About this book


Over the past decade, our laboratory and others have been concerned with molecular archaeological studies aimed at revealing the origins and evolutionary histories of permeases (1). These studies have revealed that several different families, defined on the basis of sequence similarities, arose independently of each other, at different times in evolutionary history, following different routes. When complete microbial genomes first became available for analysis, we adapted p- existing software and designed new programs that allowed us quickly to identify probable transmembrane proteins, estimate their topologies and determine the likelihood that they function in transport (2). This work allowed us to expand previously-recognized families and to identify dozens of new families. All of this work then led us to attempt to design a rational but comprehensive classification system that would be applicable to the complete complement of transport systems found in all living organisms (3). The classification system that we have devised is based primarily on mode of transport and energy coupling mechanism, secondarily on molecular phylogeny, and lastly on the substrate specificities of the individual permeases (4).


Bur DNA biochemistry enzymes evolution gene expression gene therapy genes genetic engineering genetics transcription

Editors and affiliations

  • Jane K. Setlow
    • 1
  1. 1.Brookhaven National LaboratoryUpton

Bibliographic information

  • DOI
  • Copyright Information Kluwer Academic Publishers 2001
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-306-46645-8
  • Online ISBN 978-0-306-47572-6
  • Series Print ISSN 1096-3716
  • Buy this book on publisher's site
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