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Topics in Fluorescence Spectroscopy

Principles

  • Joseph R. Lakowicz

Part of the Topics in Fluorescence Spectroscopy book series (TIFS, volume 2)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Maurice R. Eftink
    Pages 53-126
  3. Herbert C. Cheung
    Pages 127-176
  4. Martin Straume, Susan G. Frasier-Cadoret, Michael L. Johnson
    Pages 177-240
  5. Joseph M. Beechem, Enrico Gratton, Marcel Ameloot, Jay R. Knutson, Ludwig Brand
    Pages 241-305
  6. Thomas P. Burghardt, Katalin Ajtai
    Pages 307-343
  7. Richard B. Thompson
    Pages 345-365
  8. Nicolai A. Nemkovich, Anatolyi N. Rubinov, Vladimir I. Tomin
    Pages 367-428
  9. Back Matter
    Pages 429-432

About this book

Introduction

Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial two volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. The third volume will appear shortly. The first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).

Keywords

energy experiment fluorescence fluorescence spectroscopy molecule polarization quenching resonance energy sensors spectra spectroscopy

Editors and affiliations

  • Joseph R. Lakowicz
    • 1
  1. 1.Center for Fluorescence Spectroscopy Department of Biological ChemistryUniversity of Maryland School of MedicineBaltimoreUSA

Bibliographic information

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