© 2019

Methods in Rhizosphere Biology Research

  • Didier Reinhardt
  • Anil K. Sharma

Part of the Rhizosphere Biology book series (RHBIO)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Root Symbioses

    1. Front Matter
      Pages 1-1
    2. Ru-Jie Li, Yi-Han Wang, Jie Cai, Jie Liu, Zhi-Ping Xie, Christian Staehelin
      Pages 19-25
    3. Alice Drain, Laurent Bonneau, Ghislaine Recorbet, Diederik van Tuinen, Daniel Wipf, Pierre-Emmanuel Courty
      Pages 27-34
    4. Hassen Gherbi, Valérie Hocher, Mariama Ngom, Nathalie Diagne, Joëlle Fournier, Alyssa Carre-Mlouka et al.
      Pages 35-59
    5. Monica Agnolucci, Alessandra Turrini, Manuela Giovannetti
      Pages 61-79
  3. Plant Pathogens and Microbial Plant Protection

    1. Front Matter
      Pages 81-81
    2. Jacob Hargreaves, Pieter van West
      Pages 83-103
    3. Valentina Riva, Elisa Terzaghi, Lorenzo Vergani, Francesca Mapelli, Elisabetta Zanardini, Cristiana Morosini et al.
      Pages 105-132
    4. Gustavo Santoyo, Juan M. Sánchez-Yáñez, Sergio de los Santos-Villalobos
      Pages 133-149
    5. M. B. Rubio, H. A. de Medeiros, M. E. Morán-Diez, P. Castillo, R. Hermosa, E. Monte
      Pages 151-166
  4. Experimental Approaches and Analytical Techniques in Rhizosphere Biology

    1. Front Matter
      Pages 167-167
    2. Pierre Stevenel, E. Frossard, S. Abiven, I. M. Rao, F. Tamburini, A. Oberson
      Pages 169-195
    3. Yukari Kuga, Klaus Schläppi, Didier Reinhardt
      Pages 227-239
    4. Yoshihiro Kobae
      Pages 241-253
    5. Laurent Bonneau, Ghislaine Recorbet, Diederik van Tuinen, Daniel Wipf, Pierre-Emmanuel Courty
      Pages 271-279

About this book


This book compiles various methodologies used in understanding interactions within the rhizosphere. An in-depth understanding of the rhizosphere is essential to developing successful strategies for future sustainable agriculture. The book summarizes methods and techniques used to study the mechanisms involved in mutualistic symbioses and pathogenic interactions of plants with various microbial organisms including fungi, bacteria, and oomycetes. Each chapter discusses different methodologies used in rhizosphere biology, while also providing real-world experimental data and trouble-shooting tips. Interested researchers will also find a wealth of literature references for further research. As the first comprehensive manual and compilation of methods and techniques used in rhizosphere biology, the book represents an essential resource for all researchers who are newcomers to soil microbiology experimentation.


Rhizosphere Techniques Root microbiome plant-pathogen soil biology Soil biology methods

Editors and affiliations

  • Didier Reinhardt
    • 1
  • Anil K. Sharma
    • 2
  1. 1.Department of BiologyUniversity of FribourgFribourgSwitzerland
  2. 2.Biological Sciences, CBSHG.B.Pant University of Agriculture & TechnologyPantnagarIndia

About the editors

Prof. D. Reinhardt is a researcher at the University of Fribourg, Switzerland. After completing his doctoral studies in the field of plant-pathogen interactions at the University of Basel, and a postdoc stay at the Salk Institute in La Jolla (California), he worked at the University of Bern. His main area of interest encompasses various aspects of arbuscular mycorrhizal symbiosis, with particular emphasis on the genetic factors in the host required for the intracellular accommodation of the fungal symbiont, and on the regulation of AM by exogenous factors such as nutrient availability. In addition, Prof. Reinhardt is currently investigating organ formation and regulation of the body plan of plants. This includes the positioning of leaves and flowers around the growth axis, a phenomenon known as phyllotaxis. He has published over 50 research and review articles in various scientific journals. 

Prof. Anil Kumar Sharma is a Professor at the Department of Biological Sciences, CBSH G.B. of Pant University of Agriculture & Technology in Pantnagar. He completed his postdoctoral studies at the GSU, Louisiana (USA), and has been a Visiting Scientist at the University of Basel, Switzerland (2003) and University of Helsinki, Finland (2013). He has extensive research and teaching experience, and served as a reviewer for various funding bodies and international research journals. He also holds two patents in plant biology and microbiology, respectively. The recipient of various prestigious grants, he has published more than 60 research articles and 30 review articles, as well as two books with prominent publishers.

Bibliographic information

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