Immobilized β-Galactosidase-Mediated Conversion of Lactose: Process, Kinetics and Modeling Studies

  • Fadzil Noor Gonawan

Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Fadzil Noor Gonawan
    Pages 1-8
  3. Fadzil Noor Gonawan
    Pages 9-44
  4. Fadzil Noor Gonawan
    Pages 45-77
  5. Fadzil Noor Gonawan
    Pages 79-141
  6. Fadzil Noor Gonawan
    Pages 143-145
  7. Back Matter
    Pages 147-171

About this book


This book describes the reaction rate profiles of the β-galactosidase-catalyzed conversion of lactose on the inner surface of a hollow fiber membrane, which is employed as an enzymatic reactor system. The reaction rate profiles were obtained by solving the mass transfer and kinetics of reaction in a 2-dimensional model of the system. The primary challenge of this research was to develop the kinetic model of the reaction to describe the kinetic behavior as the reaction occurred on the membrane surface. Despite the difficulties, the proposed model can reliably replicate the actual process, as validation procedures have confirmed.

The reaction rates obtained analyze the performance of the immobilized enzyme on the membrane surface. Previously, an increase in performance of 'β-galactosidase-catalyzed conversion of lactose assisted by ultrafiltration was suggested due to inhibitor removal.However, as the analysis presented here shows, the concentration profile of the substrate on the membrane surface also affects the reaction performance.


Galacto-Oligosaccharides (GOS) Enzymatic Hollow Fiber Membrane Reactor (HFMR) β-Galactosidase (β-Gal) Biocatalytic Reaction Polyethyleneimine (PEI) Membrane Glutaraldehyde (GA) Trans-Membrane Pressure (TMP) Mass Transfer Model Biocatalytic Membrane Lactose Conversion

Authors and affiliations

  • Fadzil Noor Gonawan
    • 1
  1. 1.School of Chemical EngineeringUniversiti Sains MalaysiaPenangMalaysia

Bibliographic information

  • DOI
  • Copyright Information Springer Nature Singapore Pte Ltd. 2019
  • Publisher Name Springer, Singapore
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-981-13-3467-2
  • Online ISBN 978-981-13-3468-9
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • Buy this book on publisher's site
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