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Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions

  • Ang Yan Shan

Part of the Springer Theses book series (Springer Theses)

About this book

Introduction

This book provides essential insights into designing a localized DNA circuit to promote the rate of desired hybridization reactions over undesired leak reactions in the bulk solution. The area of dynamic DNA nanotechnology, or DNA circuits, holds great promise as a highly programmable toolbox that can be used in various applications, including molecular computing and biomolecular detection. However, a key bottleneck is the recurring issue of circuit leakage. The  assembly of the localized circuit is dynamically driven by the recognition of biomolecules – a different approach from most methods, which are based on a static DNA origami assembly. The design guidelines for individual reaction modules presented here, which focus on minimizing circuit leakage, are established through NUPACK simulation and tested experimentally – which  will be useful for researchers interested in adapting the concepts for other contexts. In the closing section, the design concepts are successfully applied to the biomolecular sensing of a broad range of targets including the single nucleotide mutations, proteins, and cell surface receptors. 

Keywords

DNA Computing Dynamic DNA Nanotechnology DNA Nanodevices Nupack Simulation Biomolecular Sensing Nuclei Acids Mutation Protein Surface Receptors Molecular Computing DNA Origami Assembly DNA Split Proximity Circuit

Authors and affiliations

  • Ang Yan Shan
    • 1
  1. 1.Department of Chemical and Biomolecular EngineeringNational University of SingaporeSingaporeSingapore

Bibliographic information

  • DOI https://doi.org/10.1007/978-981-13-2188-7
  • Copyright Information Springer Nature Singapore Pte Ltd. 2018
  • Publisher Name Springer, Singapore
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-981-13-2187-0
  • Online ISBN 978-981-13-2188-7
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • Buy this book on publisher's site
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