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Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

  • Bin Zhuang

Part of the Springer Theses book series (Springer Theses)

Table of contents

About this book

Introduction

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

Keywords

Integrated Module Capillary Electrophoresis Congenital Hearing Loss Composite Chip Micro-fluidic Technology DNA Extraction System PCR Microchip Automatic Genetic Analysis

Authors and affiliations

  • Bin Zhuang
    • 1
  1. 1.National Engineering Research Center for Beijing Biochip TechnologyCapitalbio Corporation BeijingChina

Bibliographic information

  • DOI https://doi.org/10.1007/978-981-10-4753-4
  • Copyright Information Springer Nature Singapore Pte Ltd. 2018
  • Publisher Name Springer, Singapore
  • eBook Packages Engineering
  • Print ISBN 978-981-10-4752-7
  • Online ISBN 978-981-10-4753-4
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • Buy this book on publisher's site
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