Radioimmunoassays for Insulin, C-Peptide and Proinsulin

  • Lise G. Heding

Table of contents

  1. Front Matter
    Pages I-6
  2. Introduction

    1. Lise G. Heding
      Pages 7-8
  3. The establishment of a radioimmunoassay

    1. Lise G. Heding
      Pages 9-13
  4. Background for development of improved and new RIAs for insulin, C-peptide and proinsulin

  5. Discussion of the methods

    1. Lise G. Heding
      Pages 16-24
  6. The present state and expected future development of methods for the determination of β-cell secretory products

  7. Summary and conclusion / Resume og konklusion

    1. Lise G. Heding
      Pages 26-28
  8. References

    1. Lise G. Heding
      Pages 29-35
  9. Original papers

  10. Back Matter
    Pages 70-83

About this book

Introduction

THE DISCOVERY OF INSULIN, C-PEPTIDE AND PROINSULlN, SPECIES DIFFERENCES Crude insulin was extracted and isolated from dog pancreases removed 7-1 ° weeks after ligation of the pancreatic duct for the first time in 1921 by Banting and Best. Its lifesaving properties were subsequently documented in pancreatectomized dogs in 1922. Only about six months elapsed from its discovery until systematic collection of calf and ox pancreases and, later, porcine pancreases was established and the blood glucose lowering effect in diabetics using the crude extract from these glands proven. It was not until 1960, however, that the primary structure of all three species of insulin, lO7 human, porcine and bovine, was elucidated . It then became evident that the differences between bovine, porcine and human insulin amounted to at mosl 3 amino acids. These apparently small differences have a significant impact on the physico-chemical characteristics of the three insulins which again affect, e.g., the rate of absorption of insulin lO9 preparations from the subcutaneous tissue and their immunogenicity, i.e.

Keywords

insulin

Authors and affiliations

  • Lise G. Heding
    • 1
  1. 1.Novo Research InstituteDenmark

Bibliographic information

  • DOI https://doi.org/10.1007/978-94-011-7094-9
  • Copyright Information Springer Science+Business Media B.V. 1988
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-94-011-7096-3
  • Online ISBN 978-94-011-7094-9
  • About this book
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