The Chloroplast: From Molecular Biology to Biotechnology

1. Chloroplast Gene Expression/Regulation

   1. Expression of Glutathione Peroxidase during Zantedeschia Aethiopica Spathe Senescence and Regreening

      • T. Lino-Neto, R. M. Tavares, K. Palme, M. S. Pais

      Pages 117-120

   2. Molecular Effects of Some Stress Factors on the Chloroplast Genetic Apparatus of the Flagellate Euglena gracilis

      • J. Krajčovič, R. Vacula, J. M. Steiner, W. Löffelhardt, A. Belicová, S. Sláviková et al.

      Pages 121-128

2. Chloroplast Envelope Membrane

   1. Front Matter

      Pages 129-129

      PDF

   2. Protein Import Across Chloroplast Envelopes

      • K. Sohrt, J. Soll

      Pages 131-136

   3. Characterization of the Arabidopsis PPI1 Mutant

      • P. Jarvis, C. Peto, J. Chory, L.-J. Chen, H.-M. Li

      Pages 137-142

   4. Isolation and Characterization of Chlamydomonas Mutants Deficient in the Plastid YCF10 Open Reading Frame

      • N. Rolland, G. Amoroso, D. Berny-Seigneurin, A.-J. Dorne, D. F. Sültemeyer, J. Joyard et al.

      Pages 143-148

   5. Structure and Function of Cema Homologue (PXCA) in Cyanobacteria

      • M. Sonoda, H. Katoh, A. Katoh, H. Ohkawa, W. Vermaas, T. Ogawa

      Pages 149-154

   6. Import and Processing of E. Coli Expressed Polyphenol Oxidase by Isolated Chloroplasts

      • S. Koussevitzky, E. Ne’eman, E. Harel

      Pages 155-160

   7. The Role of the Envelope in Assembly of Light-Harvesting Complexes in the Chloroplast: Distribution of Lhcp between Chloroplast and Vacuoles during Chloroplast Development in Chlamydomonas reinhardtii

      • Laura L. Eggink, Hyoungshin Park, J. Kenneth Hoober

      Pages 161-166

   8. Characterization of the Plastid Import Reaction of the Pea Nadph: Protochlorophyllide Oxidoreductase (POR).

      • H. Aronsson, J. Almkvist, C. Sundqvist, M. P. Timko, C. Dahlin

      Pages 167-170

   9. Tocopherol Biosynthesis in Senescing Chloroplasts - A Mechanism to Protect Envelope Membranes against Oxidative Stress and a Prerequisite for Lipid Remobilization ?

      • B. Chrost, J. Falk, B. Kernebeck, H. Mölleken, K. Krupinska

      Pages 171-176

3. Pigment Biosynthesis

   1. Front Matter

      Pages 177-177

      PDF

   2. The Effect of Light on the Biosynthesis and Function of Nadph-Protochlorophyllide Oxidoreductases (PORs) A and B in Seedlings of Arabidopsis Thaliana and Hordeum Vulgare

      • Klaus Apel, Ulrich Sperling, Gregory A. Armstrong

      Pages 179-184

   3. Redox Reactions in the Last Steps of Chlorophyll Biosynthesis

      • W. Rüdiger, H. Klement, M. Helfrich, A. Tanaka, S. Schoch, U. Oster

      Pages 185-190

   4. Cofactor Requirement of the Enzymatic Reduction of Chlorophyll B to Chlorophyll A

      • V. Scheumann, S. Schoch, W. Rüdiger

      Pages 191-194

   5. Effects of Nuclear y Mutations on Expression of Plastid Genes Required for Light-Independent Chlorophyll Formation in Chlamydomonas

      • A. B. Cahoon, M. P. Timko

      Pages 195-200

   6. Can Psilotum and/or Gnetum Synthesise Chlorophyll in Darkness?

      • J. Walmsley, H. Adamson, M. Wright, P. Wrench

      Pages 201-205

   7. Protochlorophyllide and Por in the Lip1 Mutant of Pea

      • M. Seyyedi, M. P. Timko, C. Sundqvist

      Pages 207-212

   8. Subtrate Specificity of Overexpressed Bacteriochlorophyll Synthase from Chloroflexus Aurantiacus

      • S. Schoch, U. Oster, K. Mayer, R. Feick, W. Rüdiger

      Pages 213-216

   9. Carotenoids in Higher Plants and Algae

      • Susanne Römer

      Pages 217-223

From August 10 to August 15, 1998, an international Advanced Research Workshop-Lecture Course on The chloroplast: from Molecular Biology to Biotechnology was held at the Orthodox Academy of Crete, Kolymbari-Chania, on the island of Crete, Greece. After five previous meetings on the chloroplast topic in Marburg (1975), Spetses (1978), Rhodos (1985), Aghia Pelaghia, Crete (1991) and Marburg (1995) this conference proved again that chloroplast research is continuously in the focus of intensive research interest. The meeting, sponsored by NATO and supported by the Federation of the European Societies for Plant Physiology (FESPP) and the Greek Ministry of Development (General Secretariat of Research and Development), was held under the auspices of the International Society for Chloroplast Development and the National Center for Scientific Research "Demokritos", Athens-Greece. Aim of the workshop was to bring together experts and students from different disciplines, coming from various countries around the world, studying chloroplast biogenesis from different perspectives in an effort to propose biotechnological approaches, via genetic manipulation of the organelle, applicable in solving problems of economic importance. Ninety scientists (including observers) coming from 19 countries actively participated and discussed recent advances in the field. During the meeting it became clear that as a result of the progress made in molecular biology, including genomic sequence, and in biochemistry over recent years, this exciting field of chloroplast development is continuously promoted by renewed interest in as yet unsolved but very important questions.

• Biosynthese
• Chloroplast
• Expression
• Protein
• biosynthesis
• biotechnology
• gene expression
• genes
• membrane proteins
• molecular biology
• photosynthesis
• proteins
• regulation

`It can be recommended to those graduate students and scientists already familiar with chloroplast research. Not only those having participated in this conference will appreciate this overview, but also all those who want to enter this field or want to obtain a broad information on the recent development in chloroplast research.'
Journal of Plant Physiology, 157 (2000)
`The book is a very important up-to-date summary of what is new in chloroplast research, and the individual approach of the authors in writing the chapters stamps the character of the volume. It is a fascinating high-level reading for scientists and university students interested in plant physiology, genetic, biochemistry, and molecular biology.'
Biologia Plantarum, 43:4(2000)

• Institute of Biology, NCSR “Demokritos”, Athens, Greece

  Joan H. Argyroudi-Akoyunoglou

• Fachbereich Biologie/Botanik, Philipps Universität, Marburg, Germany

  Horst Senger

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