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© 2014

ß-barrel Channel Proteins as Tools in Nanotechnology

Biology, Basic Science and Advanced Applications

  • Gives a comprehensible report on how to use beta-barrel membrane proteins as nano-material components (including all involved steps)

  • Is useful for both experimentalists and theoreticians that want to expand their knowledge toward the rapidly expanding field of membrane protein based bio-nanotechnology

  • Includes a chapter with "wet-lab" information on the beta-barrel membrane protein production (including trouble shootings and suggestions)

Book

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 794)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Marco Fioroni, Tamara Dworeck, Francisco Rodríguez-Ropero
    Pages 1-6
  3. Marco Fioroni, Tamara Dworeck, Francisco Rodríguez-Ropero
    Pages 7-40
  4. Marco Fioroni, Tamara Dworeck, Francisco Rodríguez-Ropero
    Pages 41-67
  5. Marco Fioroni, Tamara Dworeck, Francisco Rodríguez-Ropero
    Pages 69-93
  6. Marco Fioroni, Tamara Dworeck, Francisco Rodríguez-Ropero
    Pages 95-140
  7. Marco Fioroni, Tamara Dworeck, Francisco Rodríguez-Ropero
    Pages 141-163
  8. Marco Fioroni, Tamara Dworeck, Francisco Rodríguez-Ropero
    Pages 165-166
  9. Back Matter
    Pages 167-168

About this book

Introduction

b-barrel outer membrane channel proteins (OMP) have great potential as robust and flexible models or components in nanotechnology. Over the last decade biotechnological techniques allowed to expand the natural characteristics of OMPs by modifying their geometry and properties without affecting the overall protein structure and stability. The present book is oriented towards a broad group of readers including graduate students and advanced researchers. The b-barrel structure serviceability for the nano-material design will be its chief topic giving a general introduction to the field of OMP based nano-component development as well as the state of the art of the involved research. On the example of the E. coli FhuA the transformation of an OMP into a tailored nano-channel to be adapted to a non-biological synthetic (i.e. polymer) environment, rendering it competitive with artificial non-biological nano-pores will be outlined specifically the design of a set of protein nano-channels with tailored geometry (diameter, length), conductance and functionality will be reported as a case study.

In order to make this book a valuable source of information for both biotechnologists and other scientists interested in bio-nanotechnology an overview of the different steps involved in the nano-channel protein design and production will be reported. The scientific strategy from concept design, theoretical considerations, genetic engineering and large scale production, to system assembly and biophysical characterization with an overview on technological applications including membrane/polymersome technology, will be described.

Keywords

beta-barrel membrane proteins circular dichroism spectroscopy multiscale modelling nano-channels polymersomes

Authors and affiliations

  1. 1.Sustainable MomentumAachenGermany
  2. 2.Department of BiologyRWTH Aachen UniversityAachenGermany
  3. 3.Center of Smart InterfacesTechnische Universität DarmstadtDarmstadtGermany

Bibliographic information

  • Book Title ß-barrel Channel Proteins as Tools in Nanotechnology
  • Book Subtitle Biology, Basic Science and Advanced Applications
  • Authors Marco Fioroni
    Tamara Dworeck
    Francisco Rodriguez-Ropero
  • Series Title Advances in Experimental Medicine and Biology
  • Series Abbreviated Title Advs Exp. Medicine, Biology
  • DOI https://doi.org/10.1007/978-94-007-7429-2
  • Copyright Information Springer Science+Business Media Dordrecht 2014
  • Publisher Name Springer, Dordrecht
  • eBook Packages Biomedical and Life Sciences Biomedical and Life Sciences (R0)
  • Hardcover ISBN 978-94-007-7428-5
  • Softcover ISBN 978-94-024-0756-3
  • eBook ISBN 978-94-007-7429-2
  • Series ISSN 0065-2598
  • Series E-ISSN 2214-8019
  • Edition Number 1
  • Number of Pages XII, 168
  • Number of Illustrations 16 b/w illustrations, 29 illustrations in colour
  • Topics Protein Structure
    Gene Expression
    Biotechnology
    Computational Biology/Bioinformatics
  • Buy this book on publisher's site
Industry Sectors
Biotechnology

Reviews

From the reviews:

“This book is a good introduction to the problems of OMPs and their use as nanotechnology material. Because the book considers the aspects of concept design, theoretical considerations, production traps, and the biological characterization of OMP, it should be useful for graduate students and researchers in the field of nanotechnology.” (Alexander Tzanov, Computing Reviews, March, 2014)