© 2013

Regulated Proteolysis in Microorganisms

  • David A. Dougan


  • An up-to-date summary of protein degradation in microorganisms (bacteria, archaea and yeast)

  • Individual chapters by the world's leading experts in the field of regulated proteolysis

  • Detailed mechanistic analysis of the different proteolytic machines


Part of the Subcellular Biochemistry book series (SCBI, volume 66)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. AAA+ Proteolytic Machines

    1. Front Matter
      Pages 1-1
    2. Eyal Gur, Ralf Ottofueling, David A. Dougan
      Pages 3-33
    3. Eyal Gur
      Pages 35-51
  3. Regulatory Proteolysis in Bacteria

    1. Front Matter
      Pages 71-71
    2. Noël Molière, Kürşad Turgay
      Pages 73-103
    3. Dimce Micevski, David A. Dougan
      Pages 105-128
    4. Dorte Frees, Lone Brøndsted, Hanne Ingmer
      Pages 161-192
  4. Regulated Proteolysis in Yeast

    1. Front Matter
      Pages 193-193
  5. Ubiquitin-Like Protein Modification and Protein Degradation in Microorganisms

    1. Front Matter
      Pages 265-265
    2. Marie I. Samanovic, Huilin Li, K. Heran Darwin
      Pages 267-295
    3. Julie A. Maupin-Furlow
      Pages 297-327
  6. Back Matter
    Pages 329-333

About this book


This book contains an extensive collection of critical reviews, from leading researchers in the field of regulated protein degradation. It covers the role of regulated proteolysis in a range of microorganisms (from Gram positive, Gram negative and pathogenic bacteria to Archaea and the Baker’s yeast Saccharomyces cerevisiae).



For many years, intracellular protein degradation was almost exclusively thought of as a non-selective mechanism to recycle amino acids and remove trash from the cell. The contemporary view however, is that protein degradation is a highly selective process that plays a key role in a number of important cellular processes. Not only does it contribute to the maintenance of protein homeostasis (proteostasis) through the direct recognition and removal of damaged proteins, but it also regulates the cells response to a variety of environmental stress signals. Regulatory proteolysis also plays an important role in virulence of pathogenic bacteria, and “fitness” of eukaryotic organelles. This book focuses on the central players in these diverse cellular processes – the AAA+ proteases – and examines how these machines achieve their exquisite specificity. It also highlights the various mechanisms microorganisms use to post-translationally modify their proteins, as a means to regulate protein levels and hence cell physiology.




Regulated proteolysis in microorganisms is a must read for researchers working on protein homeostasis, stress response pathways and virulence. It provides a detailed overview of the components and signals that contribute to the metabolic stability of a protein in various microorganisms. As such, it also forms a useful resource for researchers in biotechnology. The book is essential reading for senior undergraduate and postgraduate students, studying all areas of protein science including protein chemistry, biochemistry, molecular biology, microbiology and biotechnology.



Key features and content:


·           Molecular mechanism of action of AAA+ proteases

·           Control of substrate specificity by adaptor proteins

·           Post-translational tagging of proteins as a means to control their metabolic stability (SsrA, Ubiquitlyation, PUPylation and SAMPylation)

·           Regulation of stress response pathways by proteolysis

·           Regulated degradation and virulence

·           Protein degradation within mitochondria


AAA+ superfamily adaptor proteins protein degradation stress response pathways

Editors and affiliations

  • David A. Dougan
    • 1
  1. 1., Department of BiochemistryLa Trobe Institute for Molecular ScienceMelbourneAustralia

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