© 2012

Isotope labeling in Biomolecular NMR

  • Hanudatta S. Atreya
  • Includes step-by-step procedures and practical tips for different isotope labeling methods

  • Covers the entire gamut of biomolecular NMR spectroscopy

  • Includes emerging areas such as structure/dynamics of large proteins, metabolomics and solid state NMR


Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 992)

Table of contents

  1. Front Matter
    Pages i-viii
  2. Proteins

    1. Front Matter
      Pages 1-1
    2. Patrik Lundström, Alexandra Ahlner, Annica Theresia Blissing
      Pages 3-15
    3. Jing Xue, David S. Burz, Alexander Shekhtman
      Pages 17-33
    4. Raffaello Verardi, Nathaniel J. Traaseth, Larry R. Masterson, Vitaly V. Vostrikov, Gianluigi Veglia
      Pages 35-62
    5. Patrik Lundström, Alexandra Ahlner, Annica Theresia Blissing
      Pages 63-82
    6. Yohei Miyanoiri, Mitsuhiro Takeda, Masatsune Kainosho
      Pages 83-93
    7. Garima Jaipuria, B. Krishnarjuna, Somnath Mondal, Abhinav Dubey, Hanudatta S. Atreya
      Pages 95-118
  3. Nucleic Acids

    1. Front Matter
      Pages 119-119
    2. Olivier Duss, Peter J. Lukavsky, Frédéric H.-T. Allain
      Pages 121-144
  4. Metabolomics

    1. Front Matter
      Pages 145-145
    2. G. A. Nagana Gowda, Narasimhamurthy Shanaiah, Daniel Raftery
      Pages 147-164
  5. Expression Systems

    1. Front Matter
      Pages 165-165
    2. Mitsuhiro Takeda, Masatsune Kainosho
      Pages 167-177
    3. Alvar D. Gossert, Wolfgang Jahnke
      Pages 179-196
    4. Mallika Sastry, Carole A. Bewley, Peter D. Kwong
      Pages 197-211
  6. Back Matter
    Pages 213-219

About this book


One of the major advancements in NMR spectroscopy of biomolecules has been the development of methods for isotope labeling. At the same time there has been a rapid progress in the number of NMR experiments that utilize such isotope labeled samples. Thus a combination of isotopic labeling and multidimensional, multinuclear experiments has significantly expanded the range of problems in structural biology amenable to NMR.

This book is intended to serve as a compendium of isotope labeling for the biomolecular NMR community providing comprehensive coverage of the existing methods and latest developments along with protocols and practical hints on the various experimental aspects. Researchers in academia or industry will find it useful to have a single book covering a wide range of topics including the emerging areas of large/membrane proteins, RNA, metabolonomics and solid state NMR.


Biomolecular NMR Genetic Engineering Isotope labeling Nucleic acids Structural Biology

Editors and affiliations

  • Hanudatta S. Atreya
    • 1
  1. 1., NMR Research CentreIndian Institute of ScienceBangaloreIndia

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