© 2013

Site-directed insertion of transgenes

  • Sylvaine Renault
  • Philippe Duchateau

Part of the Topics in Current Genetics book series (TCG, volume 23)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Site-Directed Integration of Transgenes

    1. Front Matter
      Pages 1-1
    2. Solenne Bire, Florence Rouleux-Bonnin
      Pages 3-39
  3. Integration Based on Homologous Recombination

    1. Front Matter
      Pages 69-69
    2. Judith Miné-Hattab, Rodney Rothstein
      Pages 71-89
    3. Aurélia Barascu, Anastazja Grabarz, Bernard S. Lopez
      Pages 91-120
    4. Cherie L. Ramirez, J. Keith Joung
      Pages 121-145
    5. Jean Charles Epinat, George H. Silva, Frédéric Pâques, Julianne Smith, Philippe Duchateau
      Pages 147-185
  4. Integration Based on Site-Specific Recombination

    1. Front Matter
      Pages 187-187
    2. Candice G. T. Tahimic, Kenji Sakurai, Kazuhiro Aiba, Norio Nakatsuji
      Pages 189-209
    3. Jonathan M. Geisinger, Michele P. Calos
      Pages 211-239
    4. Sean Colloms, Sylvaine Renault
      Pages 241-265
    5. Charles A. Gersbach, Carlos F. Barbas III
      Pages 267-284
  5. Applications

    1. Front Matter
      Pages 285-285
    2. Olivier Da Ines, Charles I. White
      Pages 287-315
    3. Jenny C. Barker, Richard A. Voit, Matthew H. Porteus
      Pages 327-352
    4. Christophe Delenda, Sébastien Paris, Sylvain Arnould, Edward Balbirnie, Jean-Pierre Cabaniols
      Pages 353-384
  6. Back Matter
    Pages 385-392

About this book


The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.


genome engineering homologous recombination site-specific recombination targeted genome modification transgenesis

Editors and affiliations

  • Sylvaine Renault
    • 1
  • Philippe Duchateau
    • 2
  1. 1., UFR des Sciences PharmaceutiquesUniversité François RabelaisToursFrance
  2. 2.Cellectis SAParisFrance

Bibliographic information