© 2012

Detection of Non-Amplified Genomic DNA

  • Giuseppe Spoto
  • Roberto Corradini
  • The first book dedicated to amplification-free DNA analyses

  • Presents the most cutting-edge methods and probes to analyze DNA

  • Features DNA-sequencing techniques that offer advantages for timely results, enhanced sensitivity and reduced costs

  • Each chapter offers an introduction with the general principles of the method(s) under discussion

  • Self-contained chapters provide easy access and cater for the specific interests of individual readers


Part of the Soft and Biological Matter book series (SOBIMA)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. DNA Analysis: Current Issues

    1. Front Matter
      Pages 1-1
    2. Alessia Finotti, Giulia Breveglieri, Monica Borgatti, Roberto Gambari
      Pages 3-24
    3. Rosangela Marchelli, Tullia Tedeschi, Alessandro Tonelli
      Pages 25-63
  3. Advanced Materials for Ultrasensitive Analysis

    1. Front Matter
      Pages 65-65
    2. Dong-Kwon Lim, Amit Kumar, Jwa-Min Nam
      Pages 67-87
    3. Alessandro Bertucci, Alex Manicardi, Roberto Corradini
      Pages 89-124
    4. Fengting Lv, Shu Wang
      Pages 125-150
  4. Detection Strategies – Sensors

    1. Front Matter
      Pages 151-151
    2. Di Li, Chunhai Fan
      Pages 153-183
    3. Sergio Marín, Arben Merkoçi
      Pages 185-201
    4. Roberta D’Agata, Giuseppe Spoto
      Pages 235-261
    5. Wolfgang Knoll, Jianyun Liu, Lifang Niu, Peter Eigil Nielsen, Louis Tiefenauer
      Pages 263-278
  5. Detection Strategies – Sequencing

    1. Front Matter
      Pages 279-279
    2. John F. Thompson, Fatih Ozsolak, Patrice M. Milos
      Pages 281-308
  6. Back Matter
    Pages 309-315

About this book


This book offers a state-of-the-art overview on non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. The importance of non-amplified DNA sequencing technologies will be also discussed.

Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in the field.


Enhanced sensitivity in DNA detection How to perform gene sequencing PCR-free DNA analysis

Editors and affiliations

  • Giuseppe Spoto
    • 1
  • Roberto Corradini
    • 2
  1. 1., Department of Chemical ScienceUniversity of CataniaCataniaItaly
  2. 2., Faculty of ScienceUniversity of ParmaParmaItaly

About the editors

Prof. Giuseppe Spoto, University of Catania, Catania, Italy

Prof. Roberto Corradini, University of Parma, Parma, Italy

Bibliographic information

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