Sample Preparation in Biological Mass Spectrometry

  • Alexander R. Ivanov
  • Alexander V. Lazarev

Table of contents

  1. Clinical Research And Applications

    1. Paul Dowling, Martin Clynes, Paula Meleady
      Pages 813-828
  2. Sample Preparation Techniques in Metabolomics and Drug Discovery

    1. Front Matter
      Pages 851-851
    2. Yasukazu Yoshida, Mototada Shichiri
      Pages 877-884
    3. Natalia Penner, Biplab Das, Caroline Woodward, Chandra Prakash
      Pages 885-909
  3. Sample Preparation in Analysis of Exotic and Limited Availability Specimens

    1. Front Matter
      Pages 911-911
    2. Gary B. Smejkal, George O. Poinar Jr, Pier Giorgio Righetti, Feixia Chu
      Pages 925-938
  4. Sample Treatment in Biodefense, Forensics and Infectious Diseases

    1. Front Matter
      Pages 939-939
    2. Bradford S. Powell, Robert J. Cybulski
      Pages 941-977
  5. Novel Approaches in Sample Preparation and LC-MS Analysis

    1. Front Matter
      Pages 979-979
    2. Mikhail E. Belov, Ruwan Kurulugama, Daniel Lopez-Ferrer, Yehia Ibrahim, Erin Baker
      Pages 981-1030
  6. Back Matter
    Pages 1075-1089

About this book


The goal and philosophy of sample preparation in biological mass spectrometry is to reveal the actual multicomponent molecular structure of a biological specimen that can be assessed by mass spectrometry tools. Unfortunately, sample preparation is often looked at as a necessary but “boring” step which does not warrant any efforts beyond a paragraph or two in a “Methods” section; it is believed not to be conducive to innovation, and does not elicit the enthusiasm of funding agencies. Nevertheless, in recent years, the attention to sample preparation methods has risen considerably, both in Academia and in the life science industry. The aim of this book is to provide the researcher with important sample preparation strategies for a wide variety of analyte molecules, specimens, methods, and biological applications demanding mass spectrometric analysis as the detection end-point. In this volume we have compiled contributions from several laboratories that employ mass spectrometry for biological analysis.

With the latest inventions and the introduction of highly sophisticated mass spectrometry equipment, sample preparation has become an extremely important bottleneck of biomedical analysis. This book is structured as a compilation of contributed chapters ranging from step-by-step protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.


Analytical biochemistry Mass spectrometry Proteomics and protein chemistry Protocols and novel techniques Sample preparation

Editors and affiliations

  • Alexander R. Ivanov
    • 1
  • Alexander V. Lazarev
    • 2
  1. 1., Department of Genetics and Complex DiseaHarvard School of Public Health, HarvardBostonUSA
  2. 2., Research and DevelopmentPressure BioSciences, Inc.South EastonUSA

Bibliographic information

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