Cell Line Development

  • Mohamed Al-Rubeai

Part of the Cell Engineering book series (CEEN, volume 6)

Table of contents

  1. Front Matter
    Pages i-Viii
  2. Cori Gorman, Salina Arope, Mélanie Grandjean, Pierre-Alain Girod, Nicolas Mermod
    Pages 1-32
  3. Markus O. Imhof, Philippe Chatellard, Michel Kobr, Renata Pankiewicz, Valérie Duverger, Léonard Bagnoud et al.
    Pages 33-52
  4. L. Gama-Norton, P. Riemer, U. Sandhu, K. Nehlsen, R. Schucht, H. Hauser et al.
    Pages 53-82
  5. Helen Kim, John Laudemann, Jennitte Stevens, Michelle Wu
    Pages 97-108
  6. Jennifer R. Cresswell, Nan Lin, Genova A. Richardson, Kevin J. Kayser
    Pages 109-126
  7. S. M. Browne, M. Al-Rubeai
    Pages 127-151
  8. Joseph A. Gredell, Hemant K. Kini, S. Patrick Walton
    Pages 175-194
  9. Chaya Mohan, Yeon-Gu Kim, Gyun Min Lee
    Pages 195-216
  10. Sandra V. Bennun, Frederick J. Krambeck, Michael J. Betenbaugh
    Pages 217-231
  11. Ren-Wang Peng, Martin Fussenegger
    Pages 233-248
  12. Back Matter
    Pages 249-253

About this book


Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms.

This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.


Activation CMV Expression RNA biotechnology gene expression methodology protein proteins transgen translation

Editors and affiliations

  • Mohamed Al-Rubeai
    • 1
  1. 1.Dept. Chemical & BiochemicalUniversity College, DublinBelfield, Dublin 4Ireland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 2009
  • Publisher Name Springer, Dordrecht
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-90-481-2244-8
  • Online ISBN 978-90-481-2245-5
  • Series Print ISSN 1389-6946
  • Buy this book on publisher's site
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