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Molecular Histochemical Techniques

  • Takehiko Koji
Book

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-IX
  2. Part I

    1. Front Matter
      Pages 1-1
    2. Satoshi Hashimoto, Takashi Kanematsu
      Pages 13-18
    3. Shigeyoshi Oba, Takehiko Koji
      Pages 19-30
    4. Yoshinori Otsuki, Yuko Ito
      Pages 31-46
  3. Part II

    1. Front Matter
      Pages 47-47
    2. Morimasa Matsuta, Mayumi Matsuta
      Pages 65-99
    3. Takashi Sawai, Miwa Uzuki
      Pages 100-114
    4. Hirotaka Matsumi, Tetsu Yano
      Pages 128-138
    5. Yoshio Kanemitsu, Takehiko Koji
      Pages 139-152
    6. Yoshitaka Hishikawa, Masamoto Murakami, Masao Kasahara, Naofumi Nagasue
      Pages 153-171
    7. Masanobu Miyazaki, Yoshiyuki Ozono, Takashi Harada, Shigeru Kohno
      Pages 182-195
    8. Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe
      Pages 204-221
  4. Part III

    1. Front Matter
      Pages 235-235
    2. Ko Komuta, Takashi Kanematsu, Takehiko Koji
      Pages 244-252
  5. Back Matter
    Pages 253-263

About this book

Introduction

Molecular Histochemical Techniques presents detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. In situ nick translation and TUNEL are described in detail, along with radioactive and nonradioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, a new development in molecular histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. Methodology is set forth step-by-step in easy-to-follow language by leading scientists who are working in the field today. For researchers, laboratory technicians, and graduate students, the book serves as both a rich source of the latest information and a practical lab manual.

Keywords

DNA DNA strand breaks In situ hybridization hybridization proteins transcription translation

Editors and affiliations

  • Takehiko Koji
    • 1
  1. 1.Department of Histology and Cell BiologyNagasaki UniversityNagasakiJapan

Bibliographic information

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