© 2017


Recent Analytical Techniques and Applications

  • Yasumitsu Ogra
  • Takafumi Hirata

Table of contents

  1. Front Matter
    Pages i-viii
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Hiroki Haraguchi
      Pages 3-39
  3. Analytical Techniques and Strategies Surrounding Metallomics

    1. Front Matter
      Pages 41-41
    2. Mari Shimura, Lukasz Szyrwiel, Satoshi Matsuyama, Kazuto Yamauchi
      Pages 63-92
    3. Yoshiki Makino, Seiya Ohara, Marina Yamada, Sho Mukoyama, Kentaro Hattori, Shuhei Sakata et al.
      Pages 93-106
    4. Shin-ichi Miyashita, Shin-ichiro Fujii, Kaori Shigeta, Kazumi Inagaki
      Pages 107-124
    5. Noriyuki Suzuki, Yasumitsu Ogra
      Pages 147-155
  4. Application of Metallomics in Molecular Biology, Medicine and Pharmaceutical Sciences

    1. Front Matter
      Pages 217-217
    2. Tomonari Umemura, Yuichiro Matsui, Shinnosuke Sakagawa, Taku Fukai, Eiji Fujimori, Hidetoshi Kumata et al.
      Pages 219-237
    3. Yu-ki Tanaka, Kohshiro Takata, Takahiro Kawasaki, Atsuko Shinohara, Kazuko Ishikawa-Takata, Takafumi Hirata
      Pages 239-263
    4. Seiichiro Himeno, Hitomi Fujishiro
      Pages 265-283
    5. Atsushi Takeda, Hanuna Tamano
      Pages 315-339

About this book


This book focuses on recent topics in metallomics, a study of the metallome, or metal-containing biomolecules. Metals can induce various physiological and toxicological effects in a very small amounts, in other words, the concentrations of biometals are very low in organisms. Thus, analytical techniques for a trace amount of metal are crucial to understand the biological and toxicological functions of metals.This volume begins with an overview of metallomics including the history and development of the field. Subsequent parts provide basic and advanced techniques for metallomics. Speciation and imaging of metals are basic approaches to reveal the function of the metallome. The applications of speciation using an HPLC hyphenated with inductively coupled plasma mass spectrometry (LC-ICP-MS) and flow cytometry ICP-MS are described. As advanced approaches, the applications using a micro-flow injection-ICP-MS, an ICP-triple quadrupole mass spectrometer, an ICP-sector field mass spectrometer, and an ICP-multi-collector mass spectrometer are mentioned. For the imaging of metals, basic principles and applications of several techniques such as scanning X-ray fluorescence microscopy and ICP-MS equipped with laser ablation (LA-ICP-MS) are presented. Speciation analyses using electrospray ionization mass spectrometry (ESI-MS), X-ray Absorption Spectroscopy (XAS), and nuclear magnetic resonance spectroscopy (NMR) are also introduced. The last part highlights the medical and pharmaceutical applications of metallomics. Molecular biological approaches to reveal the effects of toxic metals, metal functions in brain and neurodegenerative diseases, and metallodrugs are explained. The topic of metal transporters is also presented.


Metallomics Speciation Elemental imaging Micro-XRF NMR Stable isotope Trace elements Metal transporter Metallodrugs Brain Toxicology Neurology

Editors and affiliations

  • Yasumitsu Ogra
    • 1
  • Takafumi Hirata
    • 2
  1. 1.Graduate School of Pharmaceutical ScienceChiba UniversityChibaJapan
  2. 2.Graduate School of ScienceThe University of TokyoTokyoJapan

About the editors

Yasumitsu Ogra
Professor, Department of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, 1‐8‐1 Inohana, Chuo‐ku, Chiba 260‐8675, Japan

Takafumi Hirata
Professor, Earth and Planetary Sciences, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake‐cho, Sakyo‐ku, Kyoto 606‐8502, Japan

Bibliographic information

Industry Sectors
Health & Hospitals


“Metallomics: recent analytical techniques and applications seems adequate for Ph.D. students who are trying to approach the topic of metallomics as well as for scientists working in this discipline to update their knowledge of the field. … It provides an overview of fundamental principles, problems and tools for the analysis of biomolecules.” (Maria Montes-Bayón, Analytical and Bioanalytical Chemistry, Vol. 410, 2018)​