Advanced Methods in Structural Biology

  • Toshiya Senda
  • Katsumi Maenaka

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Protein Expression

    1. Front Matter
      Pages 1-1
    2. Hiroki Akiba, Kouhei Tsumoto
      Pages 3-23
    3. Shunsuke Kita, Katsumi Maenaka, Takashi Tadokoro
      Pages 25-45
    4. Atsushi Furukawa, Katsumi Maenaka, Takao Nomura
      Pages 67-81
    5. Takaho Terada, Seisuke Kusano, Takayoshi Matsuda, Mikako Shirouzu, Shigeyuki Yokoyama
      Pages 83-102
  3. Purification and Crystallization of Membrane Proteins

    1. Front Matter
      Pages 103-103
    2. Tomomi Kimura-Someya, Toshiaki Hosaka, Takehiro Shinoda, Kazumi Shimono, Mikako Shirouzu, Shigeyuki Yokoyama
      Pages 123-135
  4. Crystallization and Crystal Engineering

    1. Front Matter
      Pages 137-137
    2. Miki Senda, Toshiya Senda
      Pages 139-151
    3. Toshio Hakoshima
      Pages 153-161
  5. Interaction Analysis

    1. Front Matter
      Pages 163-163
    2. Elena Krayukhina, Susumu Uchiyama
      Pages 165-183
    3. Masanori Noda, Kiichi Fukui, Susumu Uchiyama
      Pages 185-198
    4. Tetsuo Ishida
      Pages 199-226
    5. Yoshihiro Kobashigawa, Natsuki Fukuda, Yusuke Nakahara, Hiroshi Morioka
      Pages 227-237
  6. Advanced Methods for Structural Analyses

    1. Front Matter
      Pages 239-239
    2. Kunio Hirata, James Foadi, Gwyndaf Evans, Kazuya Hasegawa, Oliver B. Zeldin
      Pages 241-273
    3. Kazuhiro Mio, Masahiko Sato, Chikara Sato
      Pages 275-292
    4. Tomohide Saio, Fuyuhiko Inagaki
      Pages 315-340

About this book


This book provides practical information on a whole set of protein experiments for advanced structural biology, such as X-ray crystallography, NMR, electron microscopy, advanced mass spectroscopy, and surface plasmon resonance, as well as a wide variety of expression systems including eukaryotic and in vitro expression. In the past decade, structural genomics studies have pushed forward the development of automated methods in the field of structural biology, however there is an increasing need for the structural analysis of difficult targets, such as large protein complexes and membrane proteins, which are hard to achieve using conventional automated methods, and require knowledge that goes beyond standard protein chemistry protocols. To address these problems and to help researchers develop novel methods, this volume provides examples of the development of new protein investigation methods and their theoretical background. This book particularly appeals to graduate students, postdoctoral researchers, young investigators wishing to gain a better understanding of the theory behind experiments, and those seeking further advanced, practical structural biology methods.


Protein science Experimental protocols Protein engineering Membrane proteins Drug discovery X-ray crystallography Electron microscopy NMR Surface plasmon resonance Protein expression Eukaryotic expression Mass spectrometry In vitro cell-free expression system Useful purification techniques

Editors and affiliations

  • Toshiya Senda
    • 1
  • Katsumi Maenaka
    • 2
  1. 1.Institute of Materials Structure ScienceHigh Energy Accelerator Research Organization (KEK) TsukubaJapan
  2. 2.Faculty of Pharmaceutical SciencesHokkaido UniversitySapporoJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Japan 2016
  • Publisher Name Springer, Tokyo
  • eBook Packages Springer Protocols
  • Print ISBN 978-4-431-56028-9
  • Online ISBN 978-4-431-56030-2
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
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