Patch Clamp Techniques

From Beginning to Advanced Protocols

  • Yasunobu Okada

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Fumio Kukita, Shigetoshi Oiki
    Pages 1-19
  3. Tenpei Akita, Masahiro Ohara, Yasunobu Okada
    Pages 21-41
  4. Hiromu Yawo
    Pages 43-69
  5. Hitoshi Ishibashi, Andrew J. Moorhouse, Junichi Nabekura
    Pages 71-83
  6. Harunori Ohmori
    Pages 105-119
  7. Tadashi Isa, Keiji Imoto, Yasuo Kawaguchi
    Pages 121-135
  8. Tomoyuki Takahashi, Tetsuya Hori, Yukihiro Nakamura, Takayuki Yamashita
    Pages 137-145
  9. Hiroshi Tsubokawa, Hiroto Takahashi
    Pages 159-170
  10. Hidemasa Furue
    Pages 171-182
  11. Mami Noda
    Pages 195-206
  12. Satoshi Matsuoka, Ayako Takeuchi
    Pages 207-218
  13. Minoru Horie
    Pages 219-228
  14. Takeshi Sakaba, Akaihiro Hazama, Yoshio Maruyama
    Pages 277-286
  15. Yoshio Maruyama, Akihiro Hazama
    Pages 287-293
  16. Rainer Hedrich, Dirk Becker, Dietmar Geiger, Irene Marten, M. Rob G. Roelfsema
    Pages 295-322
  17. Kohei Sawada, Takashi Yoshinaga
    Pages 323-332
  18. Seiji Hayashi, Makoto Tominaga
    Pages 333-342
  19. Kazuharu Furutani, Yoshihisa Kurachi
    Pages 353-370
  20. Ravshan Z. Sabirov, Yuri E. Korchev, Yasunobu Okada
    Pages 379-387
  21. Ravshan Z. Sabirov, Yasunobu Okada
    Pages 389-402
  22. Shigeru Morishima, Andrew F. James
    Pages 403-414
  23. Ravshan Z. Sabirov, Shigeru Morishima
    Pages 415-431
  24. Back Matter
    Pages 433-439

About this book


Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.


Biosensor Channel Patch clamp Transporter

Editors and affiliations

  • Yasunobu Okada
    • 1
  1. 1.National Institute for Physiological Sciences National Institutes of Natural SciencesOkazakiJapan

Bibliographic information

  • DOI
  • Copyright Information Springer 2012
  • Publisher Name Springer, Tokyo
  • eBook Packages Springer Protocols
  • Print ISBN 978-4-431-53992-6
  • Online ISBN 978-4-431-53993-3
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
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