© 2017

Hydrocarbon and Lipid Microbiology Protocols

Pollution Mitigation and Waste Treatment Applications

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales


  • Offers readily reproducible, step-by-step laboratory methods

  • Provides helpful tips and tricks to complement the protocols

  • Includes troubleshooting advice

  • Features practical laboratory guidelines to promote successful results


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Michael J. Firth, Roger C. Prince, Michel Boufadel
    Pages 1-10
  3. Marlea Wagelmans, Shakti Lieten
    Pages 65-79
  4. Roger C. Prince, James R. Clark, Jon E. Lindstrom
    Pages 81-93
  5. Heidi L. Gough, Jeppe L. Nielsen
    Pages 105-115
  6. Marcio Luis Busi da Silva, Jie Ma, Pedro J. J. Alvarez
    Pages 153-179
  7. Maria Nikolopoulou, Nicolas Kalogerakis
    Pages 195-220
  8. A. J. Cavaleiro, M. A. Picavet, D. Z. Sousa, A. J. M. Stams, M. A. Pereira, M. M. Alves
    Pages 221-236
  9. Odd Gunnar Brakstad, Mimmi Throne-Holst, Trond Nordtug
    Pages 237-253
  10. Esmaeil Shahsavari, Eric M. Adetutu, Andrew S. Ball
    Pages 265-279

About this book


This Volume presents applications of hydrocarbon microbiology in the context of environmental pollutant degradation, covering pollutants such as petroleum and related wastes (i.e. oil sludge), biofuels, lipid-rich wastes, chlorinated solvents and BTEX, in several environments (marine, soil, groundwater). The approaches presented range from laboratory experiments and treatment in reactors to field applications. Two chapters highlight innovative approaches to address relevant questions in pollutant degradation, such as low environmental concentrations of pollutants, and the biodegradation of complex pollutant mixtures using biofilms. Rather than presenting the applications in the form of protocols, some of the chapters in this Volume include detailed practical information on the opportunities offered by and limitations of the different approaches, providing valuable information for researchers planning to perform bioremediation experiments.

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.


Bioremediation of ground waters Bioremediation in marine environments Bioaugmentation Biostimulation of marine crude oil spills Biofuels in aquifer systems Biofilm for the remediation of oil sands Petroleum hydrocarbons contaminated soils

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.School of Biological SciencesUniversity of Essex ColchesterUnited Kingdom
  2. 2.Institute of MicrobiologyTechnical University BraunschweigBraunschweigGermany
  3. 3.Department of BiologyUniversity of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)Palma de MallorcaSpain

Bibliographic information

  • Book Title Hydrocarbon and Lipid Microbiology Protocols
  • Book Subtitle Pollution Mitigation and Waste Treatment Applications
  • Editors Terry J. McGenity
    Kenneth N. Timmis
    Balbina Nogales
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-3-662-53110-5
  • Softcover ISBN 978-3-662-57113-2
  • eBook ISBN 978-3-662-53111-2
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages X, 279
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Microbiology
    Biochemical Engineering
  • Buy this book on publisher's site
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