© 2017

Hydrocarbon and Lipid Microbiology Protocols

Meso- and Microcosms

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales
  • Offers readily reproducible, step-by-step laboratory methods

  • Provides helpful tips and tricks to complement the protocols

  • Includes troubleshooting advice

  • Features practical laboratory guidelines to promote successful results


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Roger C. Prince
    Pages 1-13
  3. Roger C. Prince, Josh D. Butler, Gail E. Bragin, Thomas F. Parkerton, Aaron D. Redman, Barbara A. Kelley et al.
    Pages 15-32
  4. Francesco Smedile, Violetta La Cono, Maria Genovese, Giovacchino Ruggeri, Renata Denaro, Francesca Crisafi et al.
    Pages 33-49
  5. Eiji Tasumi, Katsunori Yanagawa, Junichi Miyazaki, Ken Takai
    Pages 51-64
  6. Cristiana Cravo-Laureau, Ronan Jézéquel, Franck Gilbert, Philippe Cuny, Robert Duran
    Pages 79-89
  7. Philippe Cuny, Vincent Grossi, Cécile Militon, Christian Tamburini, Georges Stora, Franck Gilbert
    Pages 91-102
  8. Fanny Terrisse, Robert Duran, Isabelle Vitte, Cristiana Cravo-Laureau
    Pages 103-110
  9. Francesco Smedile, Violetta La Cono, Maria Genovese, Giovacchino Ruggeri, Renata Denaro, Francesca Crisafi et al.
    Pages 173-173

About this book


This Volume describes methods for simulating natural environments by using reproducible and controllable meso- and microcosm experiments to analyse hydrocarbon-degrading microorganisms and to test hypotheses. It presents important aspects of the preparation of experimental set-ups, hydrocarbon application and sampling, and features protocols for experiments with different types of samples, such as biofilms, aquatic systems (e.g. groundwater, streams), and sediments, including oscillating oxic-anoxic mesocosms, as well as for in-situ experimentation in subtidal and deep sediments. Two chapters are dedicated to cultivation under high-pressure conditions, and several chapters include protocols for processing samples for downstream chemical, microbial or activity analyses. Several of the approaches presented are generic and will benefit anyone embarking on designing meso- and microcosm experiments. <

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.


Volatile and non-volatile hydrocarbons Biofilms Microbe-mineral interactions Biogenic oil reservoir Crude oil-degrading bioreactors Deep-sea hydrocarbon releases

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.School of Biological SciencesUniversity of EssexColchesterUnited Kingdom
  2. 2.BraunschweigGermany
  3. 3.Dept. BiologiaUniversity of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)Palma de MallorcaSpain

Bibliographic information

  • Book Title Hydrocarbon and Lipid Microbiology Protocols
  • Book Subtitle Meso- and Microcosms
  • Editors Terry J. McGenity
    Kenneth N. Timmis
    Balbina Nogales
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-3-662-53107-5
  • Softcover ISBN 978-3-662-57112-5
  • eBook ISBN 978-3-662-53108-2
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages X, 173
  • Number of Illustrations 13 b/w illustrations, 27 illustrations in colour
  • Topics Microbiology
    Biochemical Engineering
  • Buy this book on publisher's site
Industry Sectors
Chemical Manufacturing
Health & Hospitals
Consumer Packaged Goods