© 2017

Hydrocarbon and Lipid Microbiology Protocols


  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales
  • Offers readily-reproducible step-by-step laboratory methods

  • Provides helpful tips and tricks supporting the protocols

  • Includes troubleshooting advice

  • Contains practical laboratory guidance to promote successful results


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Emma Hernandez-Sanabria, Alberto Scoma, Tim Lacoere, Marcia Duarte, Nico Boon, Dietmar H. Pieper et al.
    Pages 9-21
  3. Alberto Scoma, Emma Hernandez-Sanabria, Tim Lacoere, Howard Junca, Nico Boon, Dietmar H. Pieper et al.
    Pages 23-37
  4. Tillmann Lueders, Frederick von Netzer
    Pages 39-55
  5. Takashi Narihiro, Yuji Sekiguchi
    Pages 79-139
  6. Yue Lu, Siavash Atashgahi, Laura A. Hug, Hauke Smidt
    Pages 177-205
  7. C. J. Smith, B. A. McKew, A. Coggan, C. Whitby
    Pages 207-241

About this book


This Volume addresses the pros and cons of oligonucleotide probes, primers and primer combinations, and importantly considers how to design the best tools for the microbial taxa and/or processes being investigated. Individual chapters focus on the design of primers targeting genes that code for enzymes associated with the following functions: degradation of aromatic, aliphatic and chlorinated hydrocarbons under aerobic and anaerobic conditions, methanogenesis, methane oxidation, and the nitrogen cycle.

Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.


Methanogens Hydrocarbon degrading microbes Hydrocarbon degrading microbes Organohalide-respiring bacteria Nitrogen-cycling microbes Genes encoding enzymes for alkane degradation

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.Dept. Biological SciencesUniversity of Essex Dept. Biological SciencesColchesterUnited Kingdom
  2. 2.BraunschweigGermany
  3. 3.Department of BiologyUniversity of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)Palma de MallorcaSpain

Bibliographic information

  • Book Title Hydrocarbon and Lipid Microbiology Protocols
  • Book Subtitle Primers
  • Editors Terry J. McGenity
    Kenneth N. Timmis
    Balbina Nogales
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-3-662-50427-7
  • Softcover ISBN 978-3-662-57057-9
  • eBook ISBN 978-3-662-50428-4
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages X, 241
  • Number of Illustrations 0 b/w illustrations, 10 illustrations in colour
  • Topics Microbiology
    Biochemical Engineering
  • Buy this book on publisher's site
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