© 2016

Hydrocarbon and Lipid Microbiology Protocols

Synthetic and Systems Biology - Applications

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales Fernández


  • Offers readily-reproducible step-by-step laboratory methods

  • Provides helpful tips and tricks supporting the protocols

  • Includes troubleshooting advice

  • Contains practical laboratory guidance to promote successful results


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Yi-Shu Tai, Kechun Zhang
    Pages 9-21
  3. Claudia E. Vickers, Mareike Bongers, Sarah F. Bydder, Panagiotis Chrysanthopoulos, Mark P. Hodson
    Pages 23-52
  4. Si Jae Park, Jae Ho Shin, Jung Eun Yang, Sang Yup Lee
    Pages 53-63
  5. Till Tiso, Andrea Germer, Benjamin Küpper, Rolf Wichmann, Lars M. Blank
    Pages 65-94

About this book


This Volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology. It complements another Volume that describes generic protocols for wet experimental and computer-based systems and synthetic biology studies. The protocols in this Volume demonstrate how to employ systems and synthetic biology approaches in the design of microbes for the production of esters, isoprenoids, hydrophobic polymers, rhamnolipid biosurfactant, and peptide antimicrobial and thioether-stabilised molecules. Also presented is a protocol for the engineering of transcription factor-based biosensors for intracellular products, and another for the creation of a synthetic hydroxylase with novel activity for the selective oxyfunctionalisation of linear alkanes.

Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.


Bacteria producing esters Isoprenoid biosynthesis Alkanes Recombinant rhamnolipid Peptide antimicrobials Hydrophobic polymer production

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales Fernández
    • 3
  1. 1.Dept. Biological SciencesUniversity of EssexColchesterUnited Kingdom
  2. 2.BraunschweigGermany
  3. 3.Dept. BiologiaUniv de les Illes BalearsPalma de MallorcaSpain

Bibliographic information

  • Book Title Hydrocarbon and Lipid Microbiology Protocols
  • Book Subtitle Synthetic and Systems Biology - Applications
  • Editors Terry J. McGenity
    Kenneth N. Timmis
    Balbina Nogales Fernández
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2016
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-3-662-49126-3
  • Softcover ISBN 978-3-662-56981-8
  • eBook ISBN 978-3-662-49127-0
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages X, 137
  • Number of Illustrations 20 b/w illustrations, 20 illustrations in colour
  • Topics Microbiology
    Biochemical Engineering
  • Buy this book on publisher's site
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