Essentials of Single-Cell Analysis

Concepts, Applications and Future Prospects

  • Fan-Gang Tseng
  • Tuhin Subhra Santra

Part of the Series in BioEngineering book series (SERBIOENG)

Table of contents

  1. Front Matter
    Pages i-x
  2. Yu-Chih Chen, Patrick Ingram, Yi Luan, Euisik Yoon
    Pages 1-29
  3. Macdara Glynn, Damien King, Jens Ducrée
    Pages 31-53
  4. Tuhin Subhra Santra, Fan-Gang Tseng
    Pages 55-83
  5. Muniesh Muthaiyan Shanmugam, Tuhin Subhra Santra
    Pages 85-129
  6. Duncan Casey, Jayne Dooley
    Pages 131-157
  7. Han-Sheng Chuang, Hu-Yao Ku, Fu-Tsun Li, Aloke Kumar, Jhih-Cheng Wang, Kuan-Chih Wang
    Pages 159-194
  8. Jian Chen, Song-Bin Huang, Chengcheng Xue, Beiyuan Fan, Deyong Chen, Junbo Wang et al.
    Pages 213-234
  9. Fátima Valdés-Mora, Heather J. Lee
    Pages 257-301
  10. Hamidun Bunawan, Syarul Nataqain Baharum
    Pages 303-323
  11. Ranjita Shegokar, Sampada Sawant, Loaye Al Shaal
    Pages 325-346
  12. Rasha Abdrabu, Sudhir Kumar Sharma, Basel Khraiwesh, Kenan Jijakli, David R. Nelson, Amnah Alzahmi et al.
    Pages 363-382

About this book


This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations.

This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. 

Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan.

Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.


Continuous micro/nano-fluidic devices Electroporation Flow Cytometry Microinjection Optoporation Metabolomics Genomics and epigenomics Cell based drug delivery Single-cell sequencing Confocal Raman microscopy

Editors and affiliations

  • Fan-Gang Tseng
    • 1
  • Tuhin Subhra Santra
    • 2
  1. 1.National Tsing Hua UniversityHsinchuTaiwan
  2. 2.National Tsing Hua UniversityHsinchuTaiwan

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2016
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Engineering Engineering (R0)
  • Print ISBN 978-3-662-49116-4
  • Online ISBN 978-3-662-49118-8
  • Series Print ISSN 2196-8861
  • Series Online ISSN 2196-887X
  • Buy this book on publisher's site
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