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MicroRNA Detection and Pathological Functions

  • Xueji Zhang
  • Haifeng Dong
  • Yaping Tian

Part of the SpringerBriefs in Molecular Science book series (BRIEFSMOLECULAR)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Xueji Zhang, Haifeng Dong, Yaping Tian
    Pages 1-6
  3. Xueji Zhang, Haifeng Dong, Yaping Tian
    Pages 7-22
  4. Xueji Zhang, Haifeng Dong, Yaping Tian
    Pages 23-35
  5. Xueji Zhang, Haifeng Dong, Yaping Tian
    Pages 37-56
  6. Xueji Zhang, Haifeng Dong, Yaping Tian
    Pages 57-75
  7. Xueji Zhang, Haifeng Dong, Yaping Tian
    Pages 77-85
  8. Xueji Zhang, Haifeng Dong, Yaping Tian
    Pages 87-98
  9. Xueji Zhang, Haifeng Dong, Yaping Tian
    Pages 99-101

About this book

Introduction

This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions.

This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research.

Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China.
Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China.

Keywords

Cell imaging analysis Electrochemical genosensors Functional nanoprobe MiRNA biogenesis MiRNA detection MiRNA pathological functions Microarray hybridization Microfluidic technique Northern blotting qRT-PCR

Authors and affiliations

  • Xueji Zhang
    • 1
  • Haifeng Dong
    • 2
  • Yaping Tian
    • 3
  1. 1.Research Center for Bioengineering and Sensing TechnologyUniversity of Science & Technology BeijingBeijingChina
  2. 2.Research Center for Bioengineering and Sensing TechnologyUniversity of Science & Technology BeijingBeijingChina
  3. 3.Department of Clinical BiochemistryChinese PLA General HospitalBeijingChina

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-662-47293-4
  • Copyright Information The Author(s) 2015
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-3-662-47292-7
  • Online ISBN 978-3-662-47293-4
  • Series Print ISSN 2191-5407
  • Series Online ISSN 2191-5415
  • Buy this book on publisher's site
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