Far-Field Optical Nanoscopy

  • Philip Tinnefeld
  • Christian Eggeling
  • Stefan W. Hell

Part of the Springer Series on Fluorescence book series (SS FLUOR, volume 14)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Christian Eggeling, Stefan W. Hell
    Pages 3-25
  3. Matthew D. Lew, Steven F. Lee, Michael A. Thompson, Hsiao-lu D. Lee, W. E. Moerner
    Pages 87-110
  4. Susan Gayda, Per Niklas Hedde, Karin Nienhaus, G. Ulrich Nienhaus
    Pages 111-158
  5. Dmytro A. Yushchenko, Marcel P. Bruchez
    Pages 159-188
  6. Pedro F. Aramendía, Mariano L. Bossi
    Pages 189-213
  7. Thorben Cordes, Jan Vogelsang, Christian Steinhauer, Ingo H. Stein, Carsten Forthmann, Andreas Gietl et al.
    Pages 215-242
  8. Gert De Cremer, Bert F. Sels, Dirk E. De Vos, Johan Hofkens, Maarten B. J. Roeffaers
    Pages 245-261
  9. Maximilian H. Ulbrich
    Pages 263-291
  10. Back Matter
    Pages 331-335

About this book


This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale.

The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.


(d)STORM,(f)PALM, GSDIM Chemistry Nobel Prize® 2014 Far-field fluorescence microscopy Fluorescence labelling Fluorescent proteins NASCA Optical nanoscopy Optical super-resolution microscopy Organic dyes Photoswitchable labels STED Single-molecule tracking Stefan W. Hell William E. Moerner

Editors and affiliations

  • Philip Tinnefeld
    • 1
  • Christian Eggeling
    • 2
  • Stefan W. Hell
    • 3
  1. 1.NanoBioSciences Institute of Physical and Theoretical ChemistryTU BraunschweigBraunschweigGermany
  2. 2.MRC Human Immunology Unit and Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular MedicineUniversity of OxfordOxfordUnited Kingdom
  3. 3.Department of NanoBiophotonicsMax Planck Institute for Biophysical ChemistryGöttingenGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2015
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-3-662-45546-3
  • Online ISBN 978-3-662-45547-0
  • Series Print ISSN 1617-1306
  • Series Online ISSN 1865-1313
  • Buy this book on publisher's site
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