© 2017

Hydrocarbon and Lipid Microbiology Protocols

Isolation and Cultivation

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales


  • Offers readily reproducible, step-by-step laboratory methods

  • Provides helpful tips and tricks to complement the protocols

  • Includes troubleshooting advice

  • Features practical laboratory guidelines to promote successful results


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Yanqing Song, Bing Li, Yong Qiu, Huabing Yin
    Pages 37-55
  3. J. Cameron Thrash, Jessica Lee Weckhorst, David M. Pitre
    Pages 57-78
  4. Maria Genovese, Renata Denaro, Daniela Russo, Francesca Crisafi, Santina Santisi, Simone Cappello et al.
    Pages 79-93
  5. Lukas Y. Wick, Sally Otto, Christof Holliger
    Pages 107-117
  6. Seyed Morteza Zamir, Tina Tavassoli, Seyed Abbas Shojaosadati
    Pages 119-129
  7. Adam J. Williamson, John D. Coates
    Pages 143-164
  8. Taiki Katayama, Yoichi Kamagata
    Pages 177-195
  9. Donovan P. Kelly, Julie K. Ardley, Ann P. Wood
    Pages 197-229
  10. Svetlana N. Dedysh, Peter F. Dunfield
    Pages 231-247
  11. Marc A. Demeter, Joe Lemire, Susanne Golby, Monika Schwering, Howard Ceri, Raymond J. Turner
    Pages 249-268
  12. Dietmar Schlosser, Lukas Y. Wick
    Pages 269-281
  13. Bram Vekeman, Kim Heylen
    Pages 299-315

About this book


This Volume describes methods for cultivating hydrocarbon-producing and -consuming microbes, covering compounds in a range of states – gaseous (e.g. methane), liquid (e.g. alkanes of intermediate molecular weight) and solid (e.g. many PAHs and asphaltene). It also examines the cultivation of aerobic and anaerobic hydrocarbon degraders using a range of electron acceptors (e.g. oxygen, nitrate, sulphate, metals, (per)chlorate), and a separate chapter is devoted to explaining the cultivation of methanogens. Special attention is given to: high-throughput cultivation, growing microbes as biofilms, and cultivating fastidious microbes, as well as the preservation of microbial pure cultures and consortia. Accordingly, this Volume will be of value to anyone embarking on the selective enrichment and cultivation of novel microorganisms.

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.


Asphaltene-degrading microbes Biofilm-forming microbes Crude oil-degradation Fuel quality improvement Hydrocarbon-degrading microbes Hydrocarbons as growth substrates Methylotrophs Oil-degrading bioreactors Oil-producing microalgae

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.School of Biological SciencesUniversity of EssexColchesterUnited Kingdom
  2. 2.Institute of MicrobiologyTechnical University BraunschweigBraunschweigGermany
  3. 3.Department of BiologyUniversity of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)Palma de MallorcaSpain

Bibliographic information

  • Book Title Hydrocarbon and Lipid Microbiology Protocols
  • Book Subtitle Isolation and Cultivation
  • Editors Terry J. McGenity
    Kenneth N. Timmis
    Balbina Nogales
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-3-662-45178-6
  • Softcover ISBN 978-3-662-56878-1
  • eBook ISBN 978-3-662-45179-3
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages X, 319
  • Number of Illustrations 30 b/w illustrations, 20 illustrations in colour
  • Topics Microbiology
    Biochemical Engineering
  • Buy this book on publisher's site
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