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Chicken Egg Yolk Antibodies, Production and Application

IgY-Technology

  • Rüdiger Schade
  • Ingrid Behn
  • Michael Erhard
  • Andreas Hlinak
  • Christian Staak
Book

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages II-XII
  2. Michael Erhard, Rüdiger Schade
    Pages 1-8
  3. Andreas Hlinak, Rüdiger Schade
    Pages 9-24
  4. Christine Schwarzkopf, Christian Staak, Irene Behn, Michael Erhard
    Pages 25-64
  5. Christian Staak, Christine Schwarzkopf, Ingrid Behn, Undine Hommel, Andreas Hlinak, Rüdiger Schade et al.
    Pages 65-107
  6. Ingrid Behn, Undine Hommel, Michael Erhard, Andreas Hlinak, Rüdiger Schade, Christine Schwarzkopf et al.
    Pages 108-210
  7. Rüdiger Schade, Christine Schwarzkopf, Michael Erhard
    Pages 211-217
  8. Rüdiger Schade
    Pages 218-222
  9. Rüdiger Schade
    Pages 223-249
  10. Back Matter
    Pages 251-255

About this book

Introduction

Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals, especially rabbits. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Since hens can be kept under nearly natural conditions and antibodies be isolated from the collected eggs, this technology has become an interesting alternative to the ,bloody" techniques. For their work on yolk antibodies, the authors of this book received the FISEA prize (International Foundation for the Substitution of Animal Experimentation, Luxembourg) in 1997. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. These practical instructions are complemented by a short introduction to the hen's humoral immune system and a section on the pros and cons of chicken yolk antibodies compared to those of mammals'serum. Avian antibodies have been shown to be in some applications even more effective than mamalian antibodies, especially when phylogenetically highly conserved antigens have been used for immunisation.

Keywords

Antigen Antikörper Hühnereigelb Immunglobuline antibodies avian immune system chicken egg yolk immunoglobulin

Editors and affiliations

  • Rüdiger Schade
    • 1
  • Ingrid Behn
    • 2
  • Michael Erhard
    • 3
  • Andreas Hlinak
    • 4
  • Christian Staak
    • 5
  1. 1.Institut für Pharmakologie und ToxikologieUniversitätsklinikum Charité der Humboldt Universität zu BerlinBerlinGermany
  2. 2.Pharmazie und Psychologie Institut für ZoologieUniversität LeipzigLeipzigGermany
  3. 3.Veterinär-Physiologisches InstitutUniversität LeipzigLeipzigGermany
  4. 4.Institut für VirologieFreie Universität BerlinBerlinGermany
  5. 5.Verbraucherschutz und Veterinärmedizin (BgVV), Institut für Veterinärmedizin, FG 505Bundesinstitut für gesundheitlichenBerlinGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-662-04488-9
  • Copyright Information Springer-Verlag Berlin Heidelberg 2001
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-662-04490-2
  • Online ISBN 978-3-662-04488-9
  • Buy this book on publisher's site
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