© 1997

Plant Molecular Biology — A Laboratory Manual

  • Melody S. Clark

Part of the Springer book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XXIX
  2. Basic Molecular Techniques

    1. Front Matter
      Pages 1-1
    2. S. Wilkie, M. S. Clark, P. Leroy, M. Merlino, S. Nègre, J. C. Caissard et al.
      Pages 3-53
    3. A. Guevara-Garcia, L. Herrera-Estrella, G. Olmedo-Alvarez, M. S. Clark, G. Elgar, C.-N. Liu et al.
      Pages 54-153
    4. P. Lessard, V. Decroocq, M. Thomas, H. C. C. Foote, G. Brady, G. J. Thorlby et al.
      Pages 154-220
    5. J. R. Clark, T. Pehu
      Pages 221-277
  3. Characterization of Plant DNA

    1. Front Matter
      Pages 279-279
    2. M. Landgren, K. Glimelius
      Pages 281-304
  4. Genetic Engineering: Methodology and Analysis

    1. Front Matter
      Pages 397-397
    2. R. H. Potter, M. G. K. Jones
      Pages 399-426
    3. J. F. Topping, K. Lindsey
      Pages 427-442
    4. Y.-S. Xu, E. Pehu
      Pages 443-460
  5. Back Matter
    Pages 521-529

About this book


This manual covers the whole range of molecular biology techniques, genetic engineering as well as cytogenetics of plants. Each chapter starts with an introduction into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting. The first part of the book introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning. The second part describes analytical approaches, in particular RAPD and RFLP, while the final part encompasses a variety of gene transfer techniques and both molecular and cytological analysis. The manual will be of great use to both the first-timer and the experienced scientist.


DNA PCR RNA YAC biology cytogenetics gene gene transfer genes genetic engineering genetics molecular biology plant plant genetics plants

Editors and affiliations

  • Melody S. Clark
    • 1
  1. 1.Molecular Genetics Unit, School of Clinical Medicine, Department of Medicine, Level 5, Addenbrooke’s HospitalUniversity of CambridgeCambridgeUK

Bibliographic information

  • Book Title Plant Molecular Biology — A Laboratory Manual
  • Editors Melody S. Clark
  • Series Title Springer
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 1997
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Softcover ISBN 978-3-642-49144-3
  • eBook ISBN 978-3-642-87873-2
  • Edition Number 1
  • Number of Pages XXIX, 529
  • Number of Illustrations 40 b/w illustrations, 8 illustrations in colour
  • Topics Cell Biology
    Plant Sciences
    Biochemistry, general
  • Buy this book on publisher's site
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"Overall, this manual deserves to be highly recommended due, in particular, to the clarity of writing. ...this manual will be purchased and used by many in this rapidly expanding area of research." Heredity
"...presents a broad range of techniques needed by plant molecular biologists. The protocols are clearly presented and referenced." The New Phytologist