Hemopoietic Colonies

In Vitro Cloning of Normal and Leukemic Cells

  • Donald Metcalf

Table of contents

  1. Front Matter
    Pages i-ix
  2. Donald Metcalf
    Pages 1-4
  3. Donald Metcalf
    Pages 99-131
  4. Donald Metcalf
    Pages 140-149
  5. Donald Metcalf
    Pages 150-159
  6. Donald Metcalf
    Pages 160-169
  7. Donald Metcalf
    Pages 170-186
  8. Donald Metcalf
    Pages 187-194
  9. Donald Metcalf
    Pages 195-199
  10. Donald Metcalf
    Pages 200-205
  11. Back Matter
    Pages 206-230

About this book


The cloning of hemopoietic cells in semisolid medium began some 12 years ago, and when colonies of T = and B = lymphocytes were grown by several groups last year, the last major subclasses of hemopoietic cells had been successfully cultured in vitro. The time seemed appropriate to write a short general account of this exciting new area of hematology, emphasizing particularly the potential value of these new techniques for direct studies on patients with leukemia and allied diseases. I had hoped to refer to, or at least record, every paper published in this field, but the literature proved to be larger than I had realized, and furthermore, some areas were so active that chapters had to be rewritten over a period of less than a year. I regret that ·what follows is an incomplete account and no doubt, by the time this book becomes available, the material will be even more out of date. However, I have described the basic techniques in some detail and have tried, where possible, to explain how and why the techniques can fail. It is already possible to see patterns, perhaps even principles, emerging from this work which may well be applicable to other cell populations. These have been emphasized in the text in an attempt to make this review as creative as possible.


cell diseases hematology leukemia lymphocytes

Authors and affiliations

  • Donald Metcalf
    • 1
  1. 1.Cancer Research UnitWalter and Eliza Hall Institute of Medical ResearchMelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 1977
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-81142-5
  • Online ISBN 978-3-642-81140-1
  • Series Print ISSN 0080-0015
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Oncology & Hematology