Protein Sequence Determination

A Sourcebook of Methods and Techniques

  • Saul B. Needleman

Part of the Molecular Biology Biochemistry and Biophysics book series (MOLECULAR, volume 8)

Table of contents

  1. Front Matter
    Pages I-XVIII
  2. Saul B. Needleman
    Pages 1-4
  3. Kozo Narita, Hisayuki Matsuo, Terumi Nakajima
    Pages 30-103
  4. Hisayuki Matsuo, Kozo Narita
    Pages 104-113
  5. Ernesto Scoffone, Angelo Fontana
    Pages 162-203
  6. Pehr Edman, Agnes Henschen
    Pages 232-279
  7. Duane Gish
    Pages 322-356
  8. Back Matter
    Pages 357-396

About this book


In the few years since the first edition of this book made its appearance, under­ takings in the area of protein sequence determination have increased at an almost logarithmic pace. The logic governing such efforts has, predictably, retreated from a simple sense of curiosity in achieving something that had not previously been done, to be replaced by an absolute requirement for sequence information as the best means for understanding heretofore unattainable aspects of chemical, and structural and functional mechanistic phenomena. Witness, for example, recent volumes of the Annual Review of Biochemistry and other review journals, which treat the genetic control of mitochondrial proteins, mechanisms of enzyme action, the immunochemistry of collagen, and the role of lysosomal enzymes in the lipid storage diseases - all on the basis of known protein sequence information. One must note, too, the appearance of related works in the area of nucleic acid sequencing. The technologic advances in chemical sequencing procedures have proliferated at a remarkable rate. It is in the area of instrumentation, however, that the greatest advances have taken place. As a consequence of this, each chapter (with one single exception) in this book has been extensively revised and updated. Entire chapters have been replaced in several cases, without minimizing the value of their original content. In addition, a second volume will appear soon, dealing with subjects not covered in the present book.


Peptide Sedimentation Termination amino acid biochemistry chemistry enzyme enzymes instrumentation mitochondria molecule nucleic acid peptides protein proteins

Editors and affiliations

  • Saul B. Needleman
    • 1
    • 2
  1. 1.Scientific Affairs, Diagnostics DivisionAbbott LaboratoriesNorth ChicagoUSA
  2. 2.Departments of Biochemistry and NeurologyNorthwestern University Medical SchoolChicagoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 1975
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-80947-7
  • Online ISBN 978-3-642-80945-3
  • Series Print ISSN 0077-0221
  • Buy this book on publisher's site
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