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© 1995

Quantitation of mRNA by Polymerase Chain Reaction

Nonradioactive PCR Methods

Book

Part of the Springer Labor Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XX
  2. Theoretical and Methodical Prerequisities for Using PCR to Quantitate Nucleic Acids

  3. Conventional Techniques for mRNA Analysis

  4. Semiquantitative and Quantitative Protocols for Measurement of Nucleic Acids by PCR

  5. Back Matter
    Pages 155-165

About this book

Introduction

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research.

Keywords

Analyse DNA DNA sequencing Nucleotide PCR Quantitative PCR RNA chemical analysis chemische Analyse enzymes hybridization nucleic acid sequencing synthesis transcription

Authors and affiliations

  1. 1.Department of Medicine Institute of Clinical Chemistry and Pathological Biochemistry Molecular Biological LaboratoryUniversity of LeipzigLeipzigDeutschland
  2. 2.Department of Medicine Institute of Clinical Chemistry and Pathological Biochemistry Molecular Biological LaboratoryUniversity of LeipzigLeipzigDeutschland
  3. 3.Department of Medicine Institute of Human Genetics Researach LaboratoryUniversity of LeipzigLeipzigDeutschland
  4. 4.Department of Medicine Institute of VirologyUniversity of LeipzigLeipzigDeutschland

Bibliographic information

  • Book Title Quantitation of mRNA by Polymerase Chain Reaction
  • Book Subtitle Nonradioactive PCR Methods
  • Authors Thomas Köhler
    Dirk Laßner
    Anne-Katrin Rost
    Barbara Thamm
    Barbara Pustowoit
    Harald Remke
  • Series Title Springer Labor Manuals
  • DOI https://doi.org/10.1007/978-3-642-79712-5
  • Copyright Information Springer-Verlag Berlin Heidelberg 1995
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Hardcover ISBN 978-3-540-59192-4
  • Softcover ISBN 978-3-642-79714-9
  • eBook ISBN 978-3-642-79712-5
  • Edition Number 1
  • Number of Pages XX, 166
  • Number of Illustrations 17 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
    Analytical Chemistry
    Human Genetics
  • Buy this book on publisher's site
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Reviews

"...this technical book will be very useful for all customers of PCR. And they are numerous!... Summarizing: an excellent booklet, which we recommend highly and absolutely necessary to all practicians of PCR products and their applications." Cellular and Molecular Biology