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Quantitation of mRNA by Polymerase Chain Reaction

Nonradioactive PCR Methods

  • Thomas Köhler
  • Dirk Laßner
  • Harald Remke
  • Anne-Katrin Rost
  • Barbara Thamm
  • Barbara Pustowoit

Part of the Springer Labor Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XX
  2. Theoretical and Methodical Prerequisities for Using PCR to Quantitate Nucleic Acids

  3. Conventional Techniques for mRNA Analysis

  4. Semiquantitative and Quantitative Protocols for Measurement of Nucleic Acids by PCR

  5. Back Matter
    Pages 155-165

About this book

Introduction

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research.

Keywords

Analyse DNA DNA sequencing Nucleotide PCR Quantitative PCR RNA chemical analysis chemische Analyse enzymes hybridization nucleic acid sequencing synthesis transcription

Authors and affiliations

  • Thomas Köhler
    • 1
  • Dirk Laßner
    • 2
  • Harald Remke
    • 2
  • Anne-Katrin Rost
    • 2
  • Barbara Thamm
    • 3
  • Barbara Pustowoit
    • 4
  1. 1.Department of Medicine Institute of Clinical Chemistry and Pathological Biochemistry Molecular Biological LaboratoryUniversity of LeipzigLeipzigDeutschland
  2. 2.Department of Medicine Institute of Clinical Chemistry and Pathological Biochemistry Molecular Biological LaboratoryUniversity of LeipzigLeipzigDeutschland
  3. 3.Department of Medicine Institute of Human Genetics Researach LaboratoryUniversity of LeipzigLeipzigDeutschland
  4. 4.Department of Medicine Institute of VirologyUniversity of LeipzigLeipzigDeutschland

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-79712-5
  • Copyright Information Springer-Verlag Berlin Heidelberg 1995
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-79714-9
  • Online ISBN 978-3-642-79712-5
  • Buy this book on publisher's site
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