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Gene Transfer to Plants

  • Ingo Potrykus
  • German Spangenberg

Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XXII
  2. Agrobacterium tumefaciens-Mediated Gene Transfer

    1. Front Matter
      Pages 1-4
    2. Protocol

  3. Agrobacterium rhizogenes-Mediated Gene Transfer

  4. Direct Gene Transfer to Protoplasts

  5. Biolistic Plant Transformation

  6. Microinjection and Fiber-Mediated Transformation

    1. Front Matter
      Pages 171-175
    2. Protocol

      1. M. Schnorf, B. Kost, A. Galli, G. Neuhaus
        Pages 176-185
      2. K. Wang, B. R. Frame, P. R. Drayton, J. A. Thompson
        Pages 186-192
  7. Tissue Electroporation

  8. Analysis of Transgenic Plants

    1. Front Matter
      Pages 209-214
    2. Protocol

      1. J. Fütterer, A. Gisel, V. Iglesias, A. Klöti, B. Kost, O. Mittelsten Scheid et al.
        Pages 215-263
      2. I. Farbos, A. Mouras
        Pages 264-272
  9. Establishment and Maintenance of Embryogenic Cultures

    1. Front Matter
      Pages 291-294
    2. Protocol

  10. Genetic Markers and Expression Signals

    1. Front Matter
      Pages 305-310
    2. Protocol

      1. J. Fütterer
        Pages 311-324
      2. M. Schrott
        Pages 325-336
  11. Guidelines and Biosafety Considerations

    1. Front Matter
      Pages 337-339
    2. Protocol

      1. K. Schlüter
        Pages 350-361
  12. Back Matter
    Pages 362-362

About this book

Introduction

Using the clearly structured protocols given in this manual, it will be easy to apply the latest techniques in plant biotechnology in order to create new plant varieties or varieties with altered and optimized characteristics.
Direct gene transfer into plant cells or protoplasts by the use of microinjection, electroporation or biolistic systems, or mediated by Agrobacteria is described in detail for various plant species, including relevant crops and cereals. Also included are protocols of the standard molecular techniques for the analysis of transgenic plants as well as a section on biosafety considerations and regulations.

Keywords

Agrobacter-vermittelte Transformation Agrobacterium-mediated transformation Electrocorporation Genmanipulation Protoplasten Transg biolistic transformation biotechnology cereals gene transfer plants protoplasts tissue-electroporation transgen

Editors and affiliations

  • Ingo Potrykus
    • 1
  • German Spangenberg
    • 1
  1. 1.Swiss Federal Institute of Technology, Institute of Plant SciencesETH ZürichZürichSwitzerland

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-79247-2
  • Copyright Information Springer-Verlag Berlin Heidelberg 1995
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-48967-9
  • Online ISBN 978-3-642-79247-2
  • Buy this book on publisher's site
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