Plant Cell, Tissue and Organ Culture

1. Protoplasts and Transformation Procedures

   1. Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment

      • D. T. Tomes, M. C. Ross, D. D. Songstad

      Pages 197-213

2. Microtechniques and Analytical Procedures

   1. Front Matter

      Pages 215-215

      PDF

   2. Botanical Microtechnique for Plant Cultures

      • Larry C. Fowke, Pat J. Rennie

      Pages 217-228

   3. Transmission and Scanning Electron Microscopy for Plant Protoplasts, Cultured Cells and Tissues

      • Larry C. Fowke

      Pages 229-238

   4. Indirect Immunofluorescence Techniques for Cultured Plant Cells and Protoplasts

      • Hong Wang, Larry C. Fowke

      Pages 239-250

   5. Staining Procedures for Chromosome Analysis

      • K. C. Armstrong

      Pages 251-266

   6. Enzyme-Linked Immunosorbent Assay (ELISA) Methods to Certify Pathogen (Virus)-Free Plants

      • Adib Rowhani, Bryce W. Falk

      Pages 267-280

   7. Applications of Polymerase Chain Reaction (PCR) to Plant Genome Analysis

      • Majid R. Foolad, Siva Arulsekar, Raymond L. Rodriguez

      Pages 281-298

3. Back Matter

   Pages 299-359

   PDF

This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books.
It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

• Agrobacteria
• Agrobacterium
• Microspore
• Pflanzenzüchtung
• Polymerasekettenreaktion
• Protoplasten
• Zellkulturen
• biotechnology
• cell culture
• gene transfer
• hybrids
• micropropagation
• protoplasts
• transformation

"...provides assistance in acquiring those skills that are critical for plant tissue culture and plant biotechnology research." Horticultural Science "...a well produced book with comprehensive details on the major techniques used in plant biotechnology currently. The book is well organised and well illustrated." Australasian Biotechnology

• University of California, Davis, Belmont, USA

  Oluf L. Gamborg

• Molecular Biology Program, Department of Agronomy and Horticulture, New Mexico State University, Las Cruces, USA

  Gregory C. Phillips

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