© 1995

Plant Cell, Tissue and Organ Culture

Fundamental Methods

  • Oluf L. Gamborg
  • Gregory C. Phillips

Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XXIV
  2. Facilities and Technology Requirements for Laboratory Operations

    1. Front Matter
      Pages 1-1
    2. Oluf L. Gamborg, Gregory C. Phillips
      Pages 3-20
    3. Oluf L. Gamborg, Gregory C. Phillips
      Pages 21-34
    4. Oluf L. Gamborg, Gregory C. Phillips
      Pages 35-42
  3. Basic Plant Regeneration and Plant Propagation Procedures

    1. Front Matter
      Pages 43-43
    2. Gregory C. Phillips, John F. Hubstenberger
      Pages 45-54
    3. Gregory C. Phillips, John F. Hubstenberger, Elizabeth E. Hansen
      Pages 55-65
    4. Gregory C. Phillips, John F. Hubstenberger, Elizabeth E. Hansen
      Pages 67-79
    5. Gregory C. Phillips, John F. Hubstenberger, Elizabeth E. Hansen
      Pages 81-90
    6. John J. Finer
      Pages 91-102
  4. Applications of Organ Culture Systems

    1. Front Matter
      Pages 115-115
    2. Steven A. Slack, Laura A. Tufford
      Pages 117-128
    3. Ching-Yeh Hu, Maria Helena B. Zanettini
      Pages 129-141
    4. Timothy P. Croughan
      Pages 143-154
    5. A. M. R. Ferrie, W. A. Keller
      Pages 155-164
  5. Protoplasts and Transformation Procedures

    1. Front Matter
      Pages 165-165
    2. Stephen C. Gleddie
      Pages 167-180
    3. Ebrahim Firoozabady, Adelheid R. Kuehnle
      Pages 181-195

About this book


This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books.
It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.


Agrobacteria Agrobacterium Microspore Pflanzenzüchtung Polymerasekettenreaktion Protoplasten Zellkulturen biotechnology cell culture gene transfer hybrids micropropagation protoplasts transformation

Editors and affiliations

  • Oluf L. Gamborg
    • 1
  • Gregory C. Phillips
    • 2
  1. 1.University of California, DavisBelmontUSA
  2. 2.Molecular Biology Program, Department of Agronomy and HorticultureNew Mexico State UniversityLas CrucesUSA

Bibliographic information

  • Book Title Plant Cell, Tissue and Organ Culture
  • Book Subtitle Fundamental Methods
  • Editors Oluf Gamborg
    Gregory C. Phillips
  • Series Title Springer Lab Manual
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 1995
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Softcover ISBN 978-3-642-48974-7
  • eBook ISBN 978-3-642-79048-5
  • Edition Number 1
  • Number of Pages XXIV, 359
  • Number of Illustrations 131 b/w illustrations, 10 illustrations in colour
  • Topics Plant Sciences
    Biochemistry, general
  • Buy this book on publisher's site
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"...provides assistance in acquiring those skills that are critical for plant tissue culture and plant biotechnology research." Horticultural Science "...a well produced book with comprehensive details on the major techniques used in plant biotechnology currently. The book is well organised and well illustrated." Australasian Biotechnology