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Advanced Methods in Protein Microsequence Analysis

  • Brigitte Wittmann-Liebold
  • Johann Salnikow
  • Volker A. Erdmann

Table of contents

  1. Front Matter
    Pages I-IX
  2. Introduction

    1. Brigitte Wittmann-Liebold, Johann Salnikow, Volker A. Erdmann
      Pages 1-5
  3. Separation and Amino Acid Analysis of Proteins and Peptides for Microsequencing Studies

    1. Front Matter
      Pages 7-7
    2. Roza Maria Kamp
      Pages 8-20
    3. Heinz-Jürgen Brockmöller, Roza Maria Kamp
      Pages 34-44
  4. Manual and Solid-Phase Microsequencing Methods

    1. Front Matter
      Pages 63-63
    2. Carl C. Kuhn, John W. Crabb
      Pages 64-76
    3. Brigitte Wittmann-Liebold, Hisashi Hirano, Makoto Kimura
      Pages 77-90
    4. Werner Machleidt, Ursula Borchart, Anka Ritonja
      Pages 91-107
  5. Gas-Phase and Radio-Sequence Analysis

  6. Phenylthiohydantoin Identification, On-Line Detection, Sequences Control, and Data Processing

  7. Analysis of Cysteine Residues in Proteins

  8. Methods of Analyzing Protein Conformation

    1. Front Matter
      Pages 275-275
  9. Strategies and Specific Examples of Sequencing Proteins and Peptides

    1. Front Matter
      Pages 301-301
    2. Herwig Ponstingl, Gernot Maier, Melvyn Little, Erika Krauhs
      Pages 316-319
    3. G. Buse, G. J. Steffens, G. C. M. Steffens, L. Meinecke, S. Hensel, J. Reumkens
      Pages 340-351
  10. Alternative Sequencing Techniques for Elucidation of Peptide and Protein Structures

    1. Front Matter
      Pages 363-363
    2. Martin Digweed, Tomas Pieler, Volker A. Erdmann
      Pages 364-386
  11. Back Matter
    Pages 415-423

About this book

Introduction

Much of the recent spectacular progress in the biological sciences can be at­ tributed ot the ability to isolate, analyze, and structurally characterize proteins and peptides which are present in cells and cellular organelles in only very small amounts. Recent advances in protein chemistry and in particular the application of new micromethods have led to fruitful advances in the understanding of basic cellular processes. Areas where protein-chemical studies have resulted in interest­ ing discoveries include the peptide hormones and their release factors, growth factors and oncogenes, bioenergetics, proton pumps and ion pumps and chan­ nels, topogenesis and protein secretion, molecular virology and immunology, membrane protein analysis, and receptor research. In fact, the key methods are now on hand to unravel many of the major outstanding problems of molecular biology and in particular questions of fundamental interest which relate to devel­ opmental biology and specificity in cell-cell interaction. In this volume we have assembled descriptions of procedures which have re­ cently been shown to be efficaceous for the isolation, purification, and chemical characterization of proteins and peptides that are only available in minute amounts. Emphasis is placed on well-established micromethods which have been tested and found useful in many laboratories by experienced investigators. The chapters are written by specialists, and describe a range of sensitive techniques which can be used by researchers working in laboratories with only modest resources and equipment.

Keywords

biology cellular processes chemistry immunology membrane molecular biology oncogene oncogenes protein proteins receptor

Editors and affiliations

  • Brigitte Wittmann-Liebold
    • 1
  • Johann Salnikow
    • 2
  • Volker A. Erdmann
    • 3
  1. 1.Abteilung WittmannMax-Planck-Institut für Molekulare BiologieBerlin 33Germany
  2. 2.Institut für Biochemie und Molekulare BiologieTechnische Universität BerlinBerlin 10Germany
  3. 3.Institut für BiochemieFreie Universität BerlinBerlin 33Germany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-71534-1
  • Copyright Information Springer-Verlag Berlin Heidelberg 1986
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-71536-5
  • Online ISBN 978-3-642-71534-1
  • Buy this book on publisher's site
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