© 1986

Ultraviolet Spectroscopy of Proteins


Table of contents

  1. Front Matter
    Pages I-XI
  2. Alexander P. Demchenko
    Pages 1-4
  3. Alexander P. Demchenko
    Pages 5-26
  4. Alexander P. Demchenko
    Pages 64-90
  5. Alexander P. Demchenko
    Pages 137-144
  6. Alexander P. Demchenko
    Pages 145-172
  7. Alexander P. Demchenko
    Pages 173-182
  8. Alexander P. Demchenko
    Pages 183-197
  9. Alexander P. Demchenko
    Pages 198-207
  10. Alexander P. Demchenko
    Pages 208-221
  11. Alexander P. Demchenko
    Pages 241-262
  12. Alexander P. Demchenko
    Pages 263-278
  13. Back Matter
    Pages 279-312

About this book


The aim of this book is to give a comprehensive description of the basic methods used in the ultraviolet spectroscopy of proteins, to discuss new trends and development of these methods, and to analyze their different applications in the study of various aspects of protein structure and dynamics. Ultraviolet spectroscopy is one of the oldest and most popular methods in the field of biochemistry and molecular biophysics. At present, it is difficult to imagine the biochemical laboratory without a recording spectrophotometer or spectrofluorimeter. There are several hundreds of publications directly devoted to protein ultraviolet spectroscopy and in a great number of studies UV spectroscopic methods are used for the structural analysis of different proteins. Meanwhile a unified description of the theoretical basis of the methods, experimental techniques, data analysis, and generalization of results obtained in solving the specific problems of protein structure are lacking. There are three reasons for which a monograph on ultraviolet spectroscopy is needed today. Firstly, there has been significant growth in facilities of experimental technique, its precision, and versatility associated with computer data analysts. This new technique is available to a wide circle of scientists engaged in the field of protein research. Most of them are not spectroscopists and, thus, there is a need for a conceivable and precise source of information on how to use this method and what kind of data it should provide.


Photometer UV biochemistry biophysics protein proteins spectroscopy

Authors and affiliations

  1. 1.Palladin Institute of BiochemistryAcademy of Sciences of the UkraineKievUSSR

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