© 1980

Diffusion Chamber Culture

Hemopoiesis, Cloning of Tumors, Cytogenetic and Carcinogenic Assays

  • Eugene P. Cronkite
  • Arland L. Carsten
Conference proceedings

Table of contents

  1. Front Matter
    Pages I-XIII
  2. Characterization of Progenitor Cells in Diffusion Chambers

  3. Regulatory Mechanisms in Diffusion Chamber Cell Growth

  4. Cell Interactions, Cytogenetics, and Methodology

About these proceedings


Even though diffusion chamber culture was commenced long before ortho­ dox tissue culture by Metchnikoff (1887) there have been only sporadic attempts to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not a substitute for established in vitro culture nor for in vivo studies. It complements both. Dr. Arne B~yum introduced diffusion chamber culture at Brookhaven National Laboratory. After some modest success in showing that one could culture human bone marrow and with appropriate stimuli induce erythro­ poiesis in diffusion chambers, several of the participants at this conference visited Brookhaven to learn firsthand this simple technology and to apply it in their own laboratories. However, the technique did not spread widely and controversy arose in which the same question was repeatedly asked: Can the diffusion chamber technique provide information that is not ob­ tainable more rapidly and easily, and at less expense by the in vitro tech­ niques? As a result of our deep interest in and involvement with diffusion chamber culture Dr. A. L. Carsten and I organized this conference. A major objective of this conference was to seek answers to the above question.


Diffusion cell cytogenetics genetics leukemia mutagen tumor

Editors and affiliations

  • Eugene P. Cronkite
    • 1
  • Arland L. Carsten
    • 1
  1. 1.Medical DepartmentBrookhaven National LaboratoryUptonUSA

Bibliographic information

  • Book Title Diffusion Chamber Culture
  • Book Subtitle Hemopoiesis, Cloning of Tumors, Cytogenetic and Carcinogenic Assays
  • Editors E. P. Cronkite
    A. L. Carsten
  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 1980
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Softcover ISBN 978-3-540-10064-5
  • eBook ISBN 978-3-642-67644-4
  • Edition Number 1
  • Number of Pages XIV, 277
  • Number of Illustrations 12 b/w illustrations, 0 illustrations in colour
  • Topics Oncology
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Oncology & Hematology