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Dynamic Morphology of Leukemia Cells

A Comparative Study by Scanning Electron Microscopy and Microcinematography

  • Heidi Felix
  • Gisela Haemmerli
  • Peter Sträuli

Table of contents

  1. Front Matter
    Pages I-XIII
  2. Rat Leukemias

    1. Front Matter
      Pages 1-1
    2. Heidi Felix, Gisela Haemmerli, Peter Sträuli
      Pages 2-23
  3. Human Leukemias

    1. Front Matter
      Pages 25-25
    2. Heidi Felix, Gisela Haemmerli, Peter Sträuli
      Pages 26-175
    3. Heidi Felix, Gisela Haemmerli, Peter Sträuli
      Pages 176-177
    4. Heidi Felix, Gisela Haemmerli, Peter Sträuli
      Pages 178-180
    5. Heidi Felix, Gisela Haemmerli, Peter Sträuli
      Pages 181-184
  4. Back Matter
    Pages 185-194

About this book

Introduction

Dynamic Morphology is the attempt to correlate surface architec­ ture and shape of fixed cells, as visualized by scanning electron microscopy (SEM), with the behavior of living cells, recorded by microcinematography (MCM). If SEM and MCM are used concurrently for the analysis of cell populations, a dynamic inter­ pretation of SEM photographs is only valid if the experimental conditions are identical for the two techniques. This is achieved by allowing the cells to settle on a glass surface where they remain long enough to perform their various activities under conditions identical for both techniques (for technical details see Methodology). The analysis of a population necessitates the study of a large number of cells. This prerequisite is met by operating the scanning electron micro­ scope at low levels of magnification, and by using culture chambers for cinematography. It can be argued that the examina­ tion of attached cells excludes a complete SEM survey of a population, as cells not adhering from the outset or becoming detached during the different preparatory steps are lost. For this, cinematography proved to be a reliable control: All cell types recognized in time-lapse films were also seen in scanning electron (SE) micrographs. Another, and more general, objection to a dynamic interpretation concerns the artificiality of cellular behavior on glass. This is true, but does not invalidate compara­ tive studies making use of this substrate.

Keywords

Cells Morphology behavior cell electron microscopy leukemia methodology microscopy population

Authors and affiliations

  • Heidi Felix
    • 1
  • Gisela Haemmerli
    • 1
  • Peter Sträuli
    • 1
  1. 1.Division of Cancer Research, Institute of PathologyUniversity of ZürichZürichSwitzerland

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-66794-7
  • Copyright Information Springer-Verlag Berlin Heidelberg 1978
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-66796-1
  • Online ISBN 978-3-642-66794-7
  • Buy this book on publisher's site
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