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© 1997

Fingerprinting Methods Based on Arbitrarily Primed PCR

  • Maria Rita Micheli
  • Rodolfo Bova
Book

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XVI
  2. DNA Fingerprinting

    1. Front Matter
      Pages 1-1
    2. Maria Rita Micheli, Rodolfo Bova
      Pages 3-13
    3. DNA Extraction

      1. E. D’Ambrosio, E. Pascale
        Pages 15-20
      2. G. J. Hunt
        Pages 21-24
      3. C. Neal Stewart Jr.
        Pages 25-28
      4. G. C. Graham, R. J. Henry
        Pages 29-34
      5. J. P. Woods, D. E. Berg, K. L. Fisher
        Pages 35-40
      6. M. Bazzicalupo, S. Fancelli
        Pages 41-45
    4. Fingerprint Production

      1. R. Arribas, S. Tòrtola, J. Welsh, M. McClelland, M. A. Peinado
        Pages 47-53
      2. M. R. Micheli, R. Bova, E. D’Ambrosio
        Pages 55-63
      3. G. Caetano-Anollés
        Pages 65-80
      4. G. Caetano-Anollés
        Pages 103-117
    5. Further Protocols for Amplicon Visualization

    6. Marker Isolation and Characterization

      1. G. Caetano-Anollés
        Pages 153-160

About this book

Introduction

DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes. The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow to choose between alternative strategies. In addition to the general techniques specific research applications of particular interest are given such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumors or differentially expressed genes by RNA fingerprinting.

Keywords

DNA Fingerprinting Nucleotide PCR RNA bacteria cloning gene gene expression genome insects microorganism mutation sequencing tumors

Editors and affiliations

  • Maria Rita Micheli
    • 1
  • Rodolfo Bova
    • 2
  1. 1.Dipartimento di Biologia Cellulare e MolecolareUniversità di PerugiaPerugiaItalia
  2. 2.Dipartimento di Medicina Sperimentale e Scienze BiochimicheUniversità di Roma “Tor Vergata”RomaItalia

Bibliographic information

  • Book Title Fingerprinting Methods Based on Arbitrarily Primed PCR
  • Editors Maria R. Micheli
    Rodolfo Bova
  • Series Title Springer Lab Manuals
  • DOI https://doi.org/10.1007/978-3-642-60441-6
  • Copyright Information Springer-Verlag Berlin Heidelberg 1997
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Softcover ISBN 978-3-642-47812-3
  • eBook ISBN 978-3-642-60441-6
  • Edition Number 1
  • Number of Pages XVI, 441
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Cell Biology
    Molecular Medicine
    Biochemistry, general
  • Buy this book on publisher's site
Industry Sectors
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Reviews

"The only primer you'll ever need... great care has been taken in putting this book together, and the results are very impressive. Although the work of many authors, the contributions form a coherent and well-organized whole." Trends in Genetics
"...buy this excellent book." Cellular and Molecular Biology