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Fingerprinting Methods Based on Arbitrarily Primed PCR

  • Maria Rita Micheli
  • Rodolfo Bova

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XVI
  2. DNA Fingerprinting

    1. Front Matter
      Pages 1-1
    2. Maria Rita Micheli, Rodolfo Bova
      Pages 3-13
    3. DNA Extraction

      1. E. D’Ambrosio, E. Pascale
        Pages 15-20
      2. G. J. Hunt
        Pages 21-24
      3. C. Neal Stewart Jr.
        Pages 25-28
      4. G. C. Graham, R. J. Henry
        Pages 29-34
      5. J. P. Woods, D. E. Berg, K. L. Fisher
        Pages 35-40
      6. M. Bazzicalupo, S. Fancelli
        Pages 41-45
    4. Fingerprint Production

      1. R. Arribas, S. Tòrtola, J. Welsh, M. McClelland, M. A. Peinado
        Pages 47-53
      2. M. R. Micheli, R. Bova, E. D’Ambrosio
        Pages 55-63
      3. G. Caetano-Anollés
        Pages 65-80
      4. G. Caetano-Anollés
        Pages 103-117
    5. Further Protocols for Amplicon Visualization

    6. Marker Isolation and Characterization

      1. G. Caetano-Anollés
        Pages 153-160
      2. E. Mori, R. Fani
        Pages 161-169
      3. E. Mori, R. Fani
        Pages 171-176
    7. Research Applications

      1. R. Arribas, S. Tòrtola, J. Welsh, M. McClelland, M. A. Peinado
        Pages 177-185
      2. F. Fujimori, H. Takaya, Y. Anzai, T. Okuda
        Pages 227-241
      3. S. Fancelli, M. Bazzicalupo
        Pages 243-253
  3. RNA Fingerprinting

    1. Front Matter
      Pages 255-255
    2. Maria Rita Micheli, Rodolfo Bova
      Pages 257-268
    3. Fingerprint Production and Amplicon Retrieval

      1. M. Jørgensen, M. Bévort, N. Pallisgaard, R. Hummel, C. Hansen, M. Rohde et al.
        Pages 269-281
      2. M. Ricote, J. Welsh, M. McClelland
        Pages 283-293
      3. T. C. G. Bosch, J. Lohmann
        Pages 295-304
    4. Confirmation of Differential Expression

    5. Characterization of Differentially Expressed cDNAs

      1. C. Hansen, M. Jørgensen, M. Bévort, R. Hummel, M. Løfgreen, N. Pallisgaard et al.
        Pages 345-351
      2. A. H. Nielsen, N. Pallisgaard, H. Leffers
        Pages 359-369
    6. Research Applications

  4. Back Matter
    Pages 435-441

About this book

Introduction

DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes. The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow to choose between alternative strategies. In addition to the general techniques specific research applications of particular interest are given such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumors or differentially expressed genes by RNA fingerprinting.

Keywords

DNA Fingerprinting Nucleotide PCR RNA bacteria cloning gene gene expression genome insects microorganism mutation sequencing tumors

Editors and affiliations

  • Maria Rita Micheli
    • 1
  • Rodolfo Bova
    • 2
  1. 1.Dipartimento di Biologia Cellulare e MolecolareUniversità di PerugiaPerugiaItalia
  2. 2.Dipartimento di Medicina Sperimentale e Scienze BiochimicheUniversità di Roma “Tor Vergata”RomaItalia

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-60441-6
  • Copyright Information Springer-Verlag Berlin Heidelberg 1997
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-47812-3
  • Online ISBN 978-3-642-60441-6
  • Buy this book on publisher's site
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