© 1999

Imaging Living Cells

  • Rosario Rizzuto
  • Cristina Fasolato
  • First manual describing novel labelling strategies * Easy to follow step-by-step instructions * Practical hints and experimental strategies are given for every protocol


Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XXI
  2. The Instruments

    1. Front Matter
      Pages 1-1
    2. William T. Mason, John Hoyland, Terry J. McCann, Baggi Somasundaram, William O’Brien
      Pages 3-29
    3. Walter A. Carrington, Kevin E. Fogarty, Lawrence M. Lifshitz, Richard A. Tuft
      Pages 30-50
  3. Fluorescent Dyes

    1. Front Matter
      Pages 51-51
    2. Stephen Bolsover
      Pages 92-114
    3. Lawrence D. Robb-Gaspers, Paul A. Anderson, Andrew P. Thomas
      Pages 115-139
    4. Adam Zweifach, Markus Hoth, Richard S. Lewis
      Pages 140-163
    5. Olga Garaschuk, Arthur Konnerth
      Pages 164-189
    6. Alec W. M. Simpson, Ram V. Sharma
      Pages 190-214
    7. Ildo Nicoletti, Roberta Mannucci, Francesco Di Virgilio
      Pages 215-228
    8. Rosaria Ingrassia, Cristina Trabucchi, Andrea Bergamaschi, Antonio Malgaroli
      Pages 229-259
  4. Recombinant Probes

    1. Front Matter
      Pages 261-261
    2. Marisa Brini, Paolo Pinton, Carlo Bastianutto
      Pages 263-283
    3. Marta Murgia, Rosario Rizzuto
      Pages 327-350
    4. Francesca De Giorgi, Rosario Rizzuto
      Pages 351-361

About this book


Improved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together with classical probes. Novel labelling strategies, better software for image enhancement and analysis are now available and allow image acquisition with greater speed and precision. This lab manual, intended as for bench-top use, is suitable for both scientists and graduate students, combines an update on the most advanced imaging procedures with detailed protocols. Examples, cleverly selected from the wide repertoire of cell pyhsiology, cover different functional aspects such as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.


Calcium Calcium Dyes Confocal Microscopy GFP Path-Clamp Imaging cells cytoplasm gene expression protein

Editors and affiliations

  • Rosario Rizzuto
    • 1
  • Cristina Fasolato
    • 2
  1. 1.Department of Experimental and Diagnostic MedicineSection of General PathologyFerraraItaly
  2. 2.Department of Biomedical Sciences and CNR Center for the Study of BiomembranesUniversity of PadovaPadovaItaly

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