© 2001

Fluorescence Correlation Spectroscopy

Theory and Applications

  • First book on this important analytical technique


Part of the Springer Series in Chemical Physics book series (CHEMICAL, volume 65)

Table of contents

  1. Environmental Analysis and Monitoring

    1. Petra Schwille
      Pages 360-378
    2. Michael Brinkmeier
      Pages 379-395
    3. Joachim D. Müller, Yan Chen, Enrico Gratton
      Pages 410-437
    4. Jennifer L. Mitchell, Nancy L. Thompson
      Pages 438-458
    5. R. Rigler, S. Wennmalm, L. Edman
      Pages 459-476
  2. Back Matter
    Pages 477-489

About this book


This book presents the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. FCS is based on the detection of single molecules excited to fluorescence in diffraction limited confocal volume elements and the time correlation of stochastic events. It provides ultimate sensitivity in the analysis of molecular processes and has found numerous applications in physics, chemistry and particularly in biomolecular sciences. Its high spatial and temporal resolution has made FCS a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion and flow, as well as the physics of the excited state in solution as well as at the cellular level. Its application in high throughput drug screening is using all the potential of this prime analytical tool.


Fluorescence correlation microscopy Single molecule analysis Thermodynamic fluctuations diffraction microscopy molecule optics polarization spectroscopy stochastic phenomena and correlated motion

Authors and affiliations

  1. 1.Department of Medical BiophysicsKarolinska InstitutetStockholmSweden
  2. 2.Department of Biochemistry and Molecular Biophysics, School of MedicineWashington UniversitySt. LouisUSA

Bibliographic information