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Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

  • Thierry Rabilloud
Book

Part of the Principles and Practice book series (PRINCIPLES)

Table of contents

  1. Front Matter
    Pages I-XVI
  2. T. Rabilloud, I. Humphery-Smith
    Pages 1-8
  3. C. Monribot, H. Boucherie
    Pages 31-55
  4. T. Rabilloud, S. Charmont
    Pages 107-126
  5. L. Bini, S. Liberatori, B. Magi, B. Marzocchi, R. Raggiaschi, V. Pallini
    Pages 127-141
  6. G. L. Corthals, S. P. Gygi, R. Aebersold, S. D. Patterson
    Pages 197-231
  7. Ch. Eckerskorn, K. Strupat
    Pages 233-244
  8. Back Matter
    Pages 245-248

About this book

Introduction

Proteomics, a branch of functional genomics, can be defined as the large-scale analysis of proteins. In all these large-scale analysis processes there are three phases: (1) separation of the molecules to be analyzed; (2) characterization of the separated molecules; and (3) managing, handling and analysis of the data. This book is centered on the first two phases of proteomics, i.e. separation by two-dimensional electrophoresis and microcharacterization of the separated proteins. This choice is due to the fact that the data production phase is the major bottleneck of proteomics, while it is not the case in standard genomics. Emphasis has been placed on the rationale of each technique, so that the reader will be able to keep pace with the rapid evolution of the techniques. However, up-to-date protocols are also given to help the beginner in the field.

Keywords

2D Elektrophorese 2D electrophoresis Aminosäure-Sequenzierung Protein-Massenspektrometrie Protein-Nachweis Protein-Sequenzierung Proteome amino acid sequencing protein dectection protein mass spectrometry amino acid chemistry Elektrophorese genes genome membrane Polyacrylamid-Gelelektrophorese protein proteins Proteomics Säure sequencing Sequenzierung Termination

Editors and affiliations

  • Thierry Rabilloud
    • 1
  1. 1.CEA-Laboratoire de Bioénergétique Cellulaire et Pathologique EA 2019, DBMS/BECPGrenoble Cedex 9France

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-57105-3
  • Copyright Information Springer Verlag Berlin Heidelberg 2000
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-65792-7
  • Online ISBN 978-3-642-57105-3
  • Series Print ISSN 1866-914X
  • Series Online ISSN 1866-9158
  • Buy this book on publisher's site
Industry Sectors
Pharma
Biotechnology