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In Living Color

Protocols in Flow Cytometry and Cell Sorting

  • Rochelle A. Diamond
  • Susan Demaggio

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XXV
  2. Section 1

    1. Front Matter
      Pages 1-1
    2. Rochelle Diamond
      Pages 3-7
    3. Susan Demaggio
      Pages 9-10
  3. Section 2

    1. Front Matter
      Pages 11-11
    2. Rochelle Diamond
      Pages 13-13
    3. Susan Demaggio
      Pages 62-70
    4. David M. Coder
      Pages 76-91
    5. Rochelle A. Diamond
      Pages 92-97
  4. Section 3

    1. Front Matter
      Pages 107-107
    2. Rochelle A. Diamond
      Pages 109-109
    3. Rochelle A. Diamond, Hua Wang, Fei Chen, Mindy Wilke-Douglas
      Pages 111-141
    4. David C. Mcfarland, Gary Durack
      Pages 184-193
  5. Section 4

    1. Front Matter
      Pages 195-195
    2. Rochelle A. Diamond
      Pages 197-197
    3. M. Munn, G. Raghu, S. W. Park, C-H. Pan, A. Dayn
      Pages 267-275
    4. Mindy Wilke-Douglas, Ute Behrens-Jung
      Pages 291-301
    5. Rebecca Y. M. Poon, Betsy M. Ohlsson-Wilhelm, C. Bruce Bagwell, Katharine A. Muirhead
      Pages 302-352
  6. Section 5

    1. Front Matter
      Pages 353-353
    2. Rochelle A. Diamond
      Pages 355-355
    3. Howard M. Shapiro
      Pages 357-360
    4. Derek Davies
      Pages 361-371
    5. Derek Davies, Clare Hughes
      Pages 372-376
    6. Derek Davies, Ingrid Schmid
      Pages 377-384
    7. Derek Davies, Clare Hughes
      Pages 385-390
    8. J. Mottley, K. Yokoya, A. V. Roberts
      Pages 391-420
    9. D. Marie, N. Simon, L. Guillou, F. Partensky, D. Vaulot
      Pages 421-454
    10. Wendy Schober-Ditmore
      Pages 455-458
  7. Section 6

    1. Front Matter
      Pages 459-459
    2. Rochelle A. Diamond
      Pages 461-461
    3. Geoffrey W. Osborne, Paul Waring
      Pages 463-471
    4. Michael Fox
      Pages 472-480
    5. Michael Fox
      Pages 481-489
    6. Grant W. Meisenholder, Judy Nordberg, Roberta A. Gottlieb
      Pages 490-495

About this book

Introduction

Advances in the field of cell biology have always been closely related to the development of quantitative analytical methods that can be applied to individual cells or cell organelles. Almost from the early stages following the invention of the microscope, the investigator has been keenly interested in obtaining informa­ tion on the functionality of single cells and how cells perform under different sets of experimental conditions. Although cells could be viewed in the microscope for a few hundred years, only since the relatively recent application of autoradiography did we come to realize that, although cells may visually appear very much alike, they are quite different in their functional capacity. The quest to understand these differences in a cell population lead to a new series of techniques for labeling and quantitating DNA content and similar approaches have driven the develop­ ment of methods for analyzing various other cellular properties. The development of new analytical techniques follows the age old pattern of applying successes of the past with current inno­ vation, logic and new biological information. Results from auto­ radiography expanded the concept of the cell cycle from inter­ phase and mitosis to the more definitive GO/GI, Sand G2/M phases. This new knowledge lead to the development of techno­ logy to measure and analyze various parameters related to the cell cycle.

Keywords

Antigen Calcium DNA Endoplasmatisches Reticulum RNA Termination core facility fluoresence activated cell sorting gene expression genes hybridization migration molecular biology multiparameter immunofluorescence reporter gene expression

Editors and affiliations

  • Rochelle A. Diamond
    • 1
  • Susan Demaggio
    • 2
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadenaUSA
  2. 2.Optical Biology Core Facility Developmental Biology CenterUniversity of CaliforniaIrvineUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-57049-0
  • Copyright Information Springer-Verlag Berlin Heidelberg 2000
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-62978-5
  • Online ISBN 978-3-642-57049-0
  • Buy this book on publisher's site
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