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Methods of Preparation for Electron Microscopy

An Introduction for the Biomedical Sciences

  • David G. Robinson
  • Ulrich Ehlers
  • Rainer Herken
  • Bernd Herrmann
  • Frank Mayer
  • Friedrich-Wilhelm Schürmann

Table of contents

  1. Front Matter
    Pages I-XVII
  2. David G. Robinson, Ulrich Ehlers, Rainer Herken, Bernd Herrmann, Frank Mayer, Friedrich-Wilhelm Schürmann
    Pages 1-21
  3. David G. Robinson, Ulrich Ehlers, Rainer Herken, Bernd Herrmann, Frank Mayer, Friedrich-Wilhelm Schürmann
    Pages 23-143
  4. David G. Robinson, Ulrich Ehlers, Rainer Herken, Bernd Herrmann, Frank Mayer, Friedrich-Wilhelm Schürmann
    Pages 145-171
  5. David G. Robinson, Ulrich Ehlers, Rainer Herken, Bernd Herrmann, Frank Mayer, Friedrich-Wilhelm Schürmann
    Pages 173-180
  6. Back Matter
    Pages 181-190

About this book

Introduction

In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes­ sions had an unexpected instrument at their disposal which exceeded the reso­ lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob­ lems inherent in specimen preparation for the investigation of cellular struc­ tures. The microtechniques applied in light microscopy were no longer appli­ cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada­ tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses­ sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi­ ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.

Keywords

Thin section antibody cell cell culture electron microscopy electron optics microscopy nucleic acid resistance tissue transmission electron microscopy

Authors and affiliations

  • David G. Robinson
    • 1
  • Ulrich Ehlers
    • 1
  • Rainer Herken
    • 1
  • Bernd Herrmann
    • 1
  • Frank Mayer
    • 1
  • Friedrich-Wilhelm Schürmann
    • 1
  1. 1.Fachbereiche Biologie und MedizinUniversität GöttingenGöttingenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-48848-1
  • Copyright Information Springer-Verlag Berlin Heidelberg 1987
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-17592-6
  • Online ISBN 978-3-642-48848-1
  • Buy this book on publisher's site
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